Molecular basis for blue light-dependent phosphorylation of Arabidopsis cryptochrome 2

文献类型： 外文期刊

第一作者： Liu, Qing

作者： Liu, Qing;Wang, Qin;Deng, Weixian;Wang, Xu;Piao, Mingxin;Cai, Dawei;Li, Yaxing;Yu, Xiaolan;Oka, Yoshito;Zuo, Zecheng;Liu, Qing;Deng, Weixian;Piao, Mingxin;Zhou, Tingting;Zuo, Zecheng;Liu, Qing;Wang, Qin;Wang, Xu;Lin, Chentao;Barshop, William D.;Wohlschlegel, James;Liu, Bin

作者机构：

期刊名称：NATURE COMMUNICATIONS （ 影响因子：14.919； 五年影响因子：15.805 ）

ISSN： 2041-1723

年卷期： 2017 年 8 卷

页码：

收录情况： SCI

摘要： Plant cryptochromes undergo blue light-dependent phosphorylation to regulate their activity and abundance, but the protein kinases that phosphorylate plant cryptochromes have remained unclear. Here we show that photoexcited Arabidopsis cryptochrome 2 (CRY2) is phosphorylated in vivo on as many as 24 different residues, including 7 major phosphoserines. We demonstrate that four closely related Photoregulatory Protein Kinases (previously referred to as MUT9-like kinases) interact with and phosphorylate photoexcited CRY2. Analyses of the ppk123 and ppk124 triple mutants and amiR(4k) artificial microRNA-expressing lines demonstrate that PPKs catalyse blue light-dependent CRY2 phosphorylation to both activate and destabilize the photoreceptor. Phenotypic analyses of these mutant lines indicate that PPKs may have additional substrates, including those involved in the phytochrome signal transduction pathway. These results reveal a mechanism underlying the co-action of cryptochromes and phytochromes to coordinate plant growth and development in response to different wavelengths of solar radiation in nature.

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