Genome-wide analysis of complex wheat gliadins, the dominant carriers of celiac disease epitopes

文献类型： 外文期刊

第一作者： Wang, Da-Wei

作者： Wang, Da-Wei;Li, Da;Wang, Junjun;Zhao, Yue;Wang, Zhaojun;Yue, Guidong;Liu, Xin;Qin, Huanju;Zhang, Kunpu;Dong, Lingli;Wang, Daowen;Wang, Da-Wei;Li, Da;Wang, Junjun;Wang, Zhaojun;Wang, Daowen;Wang, Da-Wei;Wang, Daowen

作者机构：

期刊名称：SCIENTIFIC REPORTS （ 影响因子：4.379； 五年影响因子：5.133 ）

ISSN： 2045-2322

年卷期： 2017 年 7 卷

页码：

收录情况： SCI

摘要： Gliadins, specified by six compound chromosomal loci (Gli-A1/B1/D1 and Gli-A2/B2/D2) in hexaploid bread wheat, are the dominant carriers of celiac disease (CD) epitopes. Because of their complexity, genome-wide characterization of gliadins is a strong challenge. Here, we approached this challenge by combining transcriptomic, proteomic and bioinformatic investigations. Through third-generation RNA sequencing, full-length transcripts were identified for 52 gliadin genes in the bread wheat cultivar Xiaoyan 81. Of them, 42 were active and predicted to encode 25 alpha-, 11 gamma-, one delta- and five omega-gliadins. Comparative proteomic analysis between Xiaoyan 81 and six newly-developed mutants each lacking one Gli locus indicated the accumulation of 38 gliadins in the mature grains. A novel group of alpha-gliadins (the CSTT group) was recognized to contain very few or no CD epitopes. The delta-gliadins identified here or previously did not carry CD epitopes. Finally, the mutant lacking Gli-D2 showed significant reductions in the most celiac-toxic alpha-gliadins and derivative CD epitopes. The insights and resources generated here should aid further studies on gliadin functions in CD and the breeding of healthier wheat.

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