Diversification of the P genome among Agropyron Gaertn. (Poaceae) species detected by FISH

文献类型: 外文期刊

第一作者: Zhao, Yan

作者: Zhao, Yan;Wang, Junjie;Zhang, Zhong;Zhao, Yan;Wang, Junjie;Zhang, Zhong;Zhao, Yan;Wang, Junjie;Zhang, Zhong;Xie, Jihong;Dou, Quanwen

作者机构:

关键词: Agropyron;FISH;P genome;rDNA;repetitive sequence

期刊名称:COMPARATIVE CYTOGENETICS ( 影响因子:1.8; 五年影响因子:1.438 )

ISSN: 1993-0771

年卷期: 2017 年 11 卷 3 期

页码:

收录情况: SCI

摘要: The genomes of five Agropyron Gaertner, 1770 species were characterized using all potential di- or tri-nucleotide simple sequence repeat (SSR) motifs and four satellite DNA repeats as fluorescence in situ hybridization (FISH) probes. The sites of 5S and 45S rDNA were relatively conserved among the diploid and tetraploid species. A number of sites for the dinucleotide SSRs AC, AG, and pSc119.2 was detected in all investigated species except A. mongolicum Keng, 1938. Several different trinucleotide SSRs were identified in different tetraploid species. All Agropyron species were suggested to include the basic P genome, although genome differentiation was still observed. The P genome of A. mongolicum was distinct from that of the diploid A. cristatum (Linnaeus, 1753) Gaertner, 1770. and other tetraploid species, with no hybridizations for AC, AG, or pSc119.2 observed. This finding supports designation of the P genomes of A. cristatum and A. mongolicum as Pc and Pm, respectively. An exceptional 5S rDNA site revealed in one set of homoeologous chromosomes strongly supports the allopolyploid origin of A. desertorum (Fischer ex Link, 1821) Schultes, 1824. However, the diploid donors to A. desertorum need further investigation. Similarly, the unique FISH pattern of a pair of 5S rDNA-carrying chromosomes was indicative of a potential allopolyploid origin for A. fragile (Roth, 1800) Candargy, 1984. The conserved distribution of 5S and 45S rDNA suggests A. cristatum (4x) and A. michnoi Roshevitz, 1929 are closely related. Two forms of B chromosomes were identified among individuals A. mongolicum and A. desertorum plants.

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