Identification of similar transcriptional regulatory mechanisms in multiple cry genes in Bacillus thuringiensis HD12

文献类型： 外文期刊

第一作者： Sun Dong-mei

作者： Sun Dong-mei;Song Zhi-ru;Peng Qi;Shu Chang-long;Zhang Jie;Song Fu-ping

作者机构：

关键词： Bacillus thuringiensis;transcription;cry genes;insecticide

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2017 年 16 卷 1 期

页码：

收录情况： SCI

摘要： Bacillus thuringiensis subspecies morrisoni strain HD12, whose genome harbors multiple insecticidal protein-encoding genes, includes eight cry genes, as indicated by genome sequencing. This strain produces crystals that are toxic to lepidopteran species. These crystal inclusions were purified by sucrose gradients and sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SOS-PAGE), followed by liquid chromatography-mass spectrometry, and found to contain five proteins (Cry1Da, Cry1Ae, Cry1Bb, Cry1Fb, and Cry1Ja). The transcriptional activities of the cry1Da, cry1Ae, cry1Bb, cty1Fb, and cry1Ja promoters indicated that transcription of cryl Da is controlled by SigE; however, the other four cry genes were found to be controlled by both SigE and SigK. The activities of the cry-1Ja and cry1Fb promoters were the strongest among the five genes studied. These promoters were therefore used to direct the expression of the Cry1Ac- and Cry2Ab-encoding genes concurrently in a single strain. Our findings indicate that promoters with the same transcriptional profile may be used to direct the expression of different cry genes in one strain. Our results are expected to be valuable for the construction of strains with efficient expression of multiple cry genes in order to overcome current limitations associated with the application of B. thuringiensis-based insecticides.

分类号：

  • 相关文献

[1]Target site insensitivity mutations in the AChE and LdVssc1 confer resistance to pyrethroids and carbamates in Leptinotarsa decemlineata in northern Xinjiang Uygur autonomous region. Jiang, Wei-Hua,Lu, Wei-Ping,Shi, Xiao-Qin,Xiong, Man-Hui,Wang, Zhi-Tian,Li, Guo-Qing,Guo, Wen-Chao.

[2]Effects of pyrethroids on neuronal excitability of adult honeybees Apis mellifera. Zhang, Yi-Li,Sun, Ji-Hu,Zhou, Ting,Zhou, Wei,Wang, Qiang,Dai, Ping-Li,Liu, Feng,Zhou, Ting,Zhou, Wei,Wang, Qiang,Dai, Ping-Li.

[3]APPLICATION OF OLIGONUCLEOTIDE MICROARRAY FOR THE DETECTION AND GENOTYPING OF CRY GENES IN Bacillus thuringiensis. Liu Xu-Guang,Song Fu-Ping,Zhang Jie,Liu Xu-Guang,Wen Si-Yuan,Wang Sheng-Qi,Huang Da-Fang. 2010

[4]Toxicity of chlorantraniliprole to Cry1Ac-susceptible and resistant strains of Helicoverpa armigera. Lu, Qiong,Zhang, Lili,Guo, Fang,Liang, Gemei,Wu, Kongming,Guo, Yuyuan,Wyckhuys, Kris A. G..

[5]Increased expression of CSP and CYP genes in adult silkworm females exposed to avermectins. Xuan, Ning,Guo, Xia,Xie, Hong-Yan,Liu, Guo-Xia,Picimbon, Jean-Francois,Lou, Qi-Nian,Lu, Xing-Bo. 2015

[6]Using Next-Generation Sequencing to Detect Differential Expression Genes in Bradysia odoriphaga after Exposure to Insecticides. Chen, Haoliang,Lin, Lulu,Ali, Farman,Xie, Minghui,Zhang, Guangling,Su, Weihua,Ali, Farman. 2017

[7]Identification of glutathione S-transferases in Bemisia tabaci (Hemiptera: Aleyrodidae) and evidence that GSTd7 helps explain the difference in insecticide susceptibility between B.tabaci Middle East-Minor Asia 1 and Mediterranean. He, C.,He, C.,Xie, W.,Yang, X.,Wang, S. -L.,Wu, Q. -J.,Zhang, Y. -J.. 2018

[8]Identification and biochemical characterization of Laodelphax striatellus neutral ceramidase. Zhou, Y.,Lin, X. -W.,Zhang, Y. -R.,Huang, Y. -J.,Zhang, C. -H.,Yang, Q.,Yuan, J. -Q.,Cheng, J. -A.,Zhu, Z. -R.,Zhou, Y.,Lin, X. -W.,Zhang, Y. -R.,Huang, Y. -J.,Zhang, C. -H.,Yang, Q.,Yuan, J. -Q.,Cheng, J. -A.,Zhu, Z. -R.,Zhou, Y.,Xu, R.,Mao, C.,Li, H. -Y.,Yang, Q.. 2013

[9]Sublethal Effects of Insecticide Exposure on Megacopta cribraria (Fabricius) Nymphs: Key Biological Traits and Acetylcholinesterase Activity. Miao, Jin,Li, Guoping,Wu, Yuqing,Miao, Jin,Reisig, Dominic D.,Li, Guoping. 2016

[10]Joint toxicity of chlorpyrifos, atrazine, and cadmium at lethal concentrations to the earthworm Eisenia fetida. Yang, Guiling,Wang, Yanhua,Cai, Leiming,Wang, Qiang,Chen, Chen,Kong, Xiangzhen,Qian, Yongzhong.

[11]Recent progress of highly efficient in vivo biological screening for novel agrochemicals in China. Qian, Xuhong,Li, Baoju,Yuan, Huizhu,Fang, Jichao,Tao, Liming,Fan, Zhijin.

[12]The efficacies of 5 insecticides against hard ticks Hyalomma asiaticum, Haemaphysalis longicornis and Rhipicephalus sanguineus. Lu, Haiyan,Ren, Qiaoyun,Li, Youquan,Liu, Junlong,Niu, Qingli,Yin, Hong,Guan, Guiquan,Luo, Jianxun,Yin, Hong,Lu, Haiyan,Meng, Qingling.

[13]Mineralization of chlorpyrifos by co-culture of Serratia and Trichosporon spp.. Xu, Gangming,Li, Yingying,Zheng, Wei,Peng, Xiang,Li, Wen,Yan, Yanchun.

[14]Demographic Changes in Multigeneration Plutella xylostella (Lepidoptera: Plutellidae) After Exposure to Sublethal Concentrations of Spinosad. Yin, Xian-Hui,Wu, Qing-Jun,Zhang, You-Jun,Xu, Bao-Yun,Yin, Xian-Hui,Li, Xue-Feng,Wu, Qing-Jun.

[15]Effect of Six Insecticides on Three Populations of Bactrocera (Tetradacus) minax (Diptera: Tephritidae). Liu, Haoqiang,Jiang, Gaofei,Zhang, Yunfei,Chen, Fei,Li, Xiaojiao,Yue, Jiansu,Ran, Chun,Jiang, Gaofei,Chen, Fei,Zhao, Zhimo,Zhang, Yunfei,Li, Xiaojiao,Yue, Jiansu.

[16]Insecticide resistance in Bemisia tabaci biotype Q (Hemiptera: Aleyrodidae) from China. Luo, C.,Jones, C. M.,Devine, G.,Denholm, I.,Gorman, K.,Luo, C.,Zhang, F..

[17]Ternary toxicological interactions of insecticides, herbicides, and a heavy metal on the earthworm Eisenia fetida. Wang, Yanhua,Zhao, Xueping,Wang, Qiang,Chen, Chen,Qian, Yongzhong.

[18]Effect of chlorpyrifos on infectivity and survival of Steinernema feltiae. Chen, SL,Han, XY,Moens, M. 2003

[19]The growth and antioxidant defense responses of wheat seedlings to omethoate stress. Liang, Yongchao,Zhang, Bo,Chu, Guixin,Wei, Changzhou,Ye, Jun,Li, Zhiqiang,Zhang, Bo.

[20]Effect of heat shock on the susceptibility of Frankliniella occidentalis (Thysanoptera: Thripidae) to insecticides. Zuo Tai-qiang,Sun Li-juan,Wang Si-fang,Zheng Chang-ying,Wan Fang-hao,Wan Fang-hao,Li Hong-gang. 2016

作者其他论文 更多>>

微信小程序