Identification of similar transcriptional regulatory mechanisms in multiple cry genes in Bacillus thuringiensis HD12

文献类型： 外文期刊

第一作者： Sun Dong-mei

作者： Sun Dong-mei;Song Zhi-ru;Peng Qi;Shu Chang-long;Zhang Jie;Song Fu-ping

作者机构：

关键词： Bacillus thuringiensis;transcription;cry genes;insecticide

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2017 年 16 卷 1 期

页码：

收录情况： SCI

摘要： Bacillus thuringiensis subspecies morrisoni strain HD12, whose genome harbors multiple insecticidal protein-encoding genes, includes eight cry genes, as indicated by genome sequencing. This strain produces crystals that are toxic to lepidopteran species. These crystal inclusions were purified by sucrose gradients and sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SOS-PAGE), followed by liquid chromatography-mass spectrometry, and found to contain five proteins (Cry1Da, Cry1Ae, Cry1Bb, Cry1Fb, and Cry1Ja). The transcriptional activities of the cry1Da, cry1Ae, cry1Bb, cty1Fb, and cry1Ja promoters indicated that transcription of cryl Da is controlled by SigE; however, the other four cry genes were found to be controlled by both SigE and SigK. The activities of the cry-1Ja and cry1Fb promoters were the strongest among the five genes studied. These promoters were therefore used to direct the expression of the Cry1Ac- and Cry2Ab-encoding genes concurrently in a single strain. Our findings indicate that promoters with the same transcriptional profile may be used to direct the expression of different cry genes in one strain. Our results are expected to be valuable for the construction of strains with efficient expression of multiple cry genes in order to overcome current limitations associated with the application of B. thuringiensis-based insecticides.

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