Combined transcriptome and metabolome analyses to understand the dynamic responses of rice plants to attack by the rice stem borer Chilo suppressalis (Lepidoptera: Crambidae)

文献类型： 外文期刊

第一作者： Wang, Xingyun

作者： Wang, Xingyun;Romeis, Jorg;Peng, Yufa;Li, Yunhe;Tzin, Vered;Romeis, Jorg

作者机构：

关键词： Oryza sativa;Induced response;Next generation sequencing;Plant-insect interactions;Phytohormones;Phenylpropanoids;Carbohydrates;Amino acids;Terpenoids

期刊名称：BMC PLANT BIOLOGY （ 影响因子：4.215； 五年影响因子：4.96 ）

ISSN： 1471-2229

年卷期： 2016 年 16 卷

页码：

收录情况： SCI

摘要： Background: Rice (Oryza sativa L.), which is a staple food for more than half of the world's population, is frequently attacked by herbivorous insects, including the rice stem borer, Chilo suppressalis. C. suppressalis substantially reduces rice yields in temperate regions of Asia, but little is known about how rice plants defend themselves against this herbivore at molecular and biochemical level. Results: In the current study, we combined next-generation RNA sequencing and metabolomics techniques to investigate the changes in gene expression and in metabolic processes in rice plants that had been continuously fed by C. suppressalis larvae for different durations (0, 24, 48, 72, and 96 h). Furthermore, the data were validated using quantitative real-time PCR. There were 4,729 genes and 151 metabolites differently regulated when rice plants were damaged by C. suppressalis larvae. Further analyses showed that defense-related phytohormones, transcript factors, shikimate-mediated and terpenoid-related secondary metabolism were activated, whereas the growth-related counterparts were suppressed by C. suppressalis feeding. The activated defense was fueled by catabolism of energy storage compounds such as monosaccharides, which meanwhile resulted in the increased levels of metabolites that were involved in rice plant defense response. Comparable analyses showed a correspondence between transcript patterns and metabolite profiles. Conclusion: The current findings greatly enhance our understanding of the mechanisms of induced defense response in rice plants against C. suppressalis infestation at molecular and biochemical levels, and will provide clues for development of insect-resistant rice varieties.

分类号：

  • 相关文献

[1]Identification of Conserved and Diverse Metabolic Shift of the Stylar, Intermediate and Peduncular Segments of Cucumber Fruit during Development. Hu, Chaoyang,Zhao, Huiyu,Wang, Wen,Xu, Mingfei,Yang, Guiling,Hu, Chaoyang,Shi, Jianxin,Nie, Xiangbo. 2018

[2]Soil C and N pools in patchy shrublands of the Negev and Chihuahuan Deserts. Thompson, Thomas L.,Zaady, Eli,Huancheng, Pang,Wilson, Thomas B.,Martens, Dean A.. 2006

[3]The 3,000 rice genomes project. Rellosa, Myla Christy. 2014

[4]Plant genotypes affect aboveground and belowground herbivore interactions by changing chemical defense. Huang, Wei,Ding, Jianqing,Li, Xiaoqiong,Wen, Yuanguang,Guo, Wenfeng,Siemann, Evan.

[5]Chemical Constituents and Biological Activities of Plants from the Genus Ligustrum. She, Gai-Mei,Gao, Bei-Bei,She, Dong-Mei. 2013

[6]Largely different contents of terpenoids in beef red-flesh tangerine and its wild type. Li, Wenyun,He, Min,Xu, Juan,Li, Wenyun,Li, Jinqiang,Cai, Yongqiang,Ma, Yuhua,Liu, Cuihua. 2017

[7]Allelopathic effects of Parthenium hysterophorus L. volatiles and its chemical components. Chen, Yebing,Wang, Jinxin,Wu, Xiaohu,Sun, Jian,Yang, Na,Chen, Yebing.

[8]Chlorophyll Content and Chlorophyll Fluorescence in Tomato Leaves Infested With an Invasive Mealybug, Phenacoccus solenopsis (Hemiptera: Pseudococcidae). Huang, Jun,Zhang, Juan,Li, Ming-Jiang,Zhang, Peng-Jun,Lu, Yao-Bin,Huang, Fang. 2013

[9]Plant defense responses induced by Bemisia tabaci Middle EastAsia Minor 1 salivary components. Yan, Ying,Zhang, HaiJing,Yang, YiTing,Zhang, Yan,Guo, JianYang,Liu, WanXue,Wan, FangHao,Wan, FangHao.

[10]Plant defence negates pathogen manipulation of vector behaviour. Liu, Baiming,Shi, Xiaobin,Wu, Huaitong,Xie, Wen,Wang, Shaoli,Wu, Qingjun,Zhang, Youjun,Liu, Baiming,Preisser, Evan L.,Li, Chuanyou,Li, Chuanyou.

[11]Transcriptome analysis of the germinated seeds identifies low-temperature responsive genes involved in germination process in Ricinus communis. Wang, Xin,Wang, Lijun,Yan, Xingchu,Wang, Lei,Tan, Meilian,Geng, Xinxin,Wei, Wenhui,Wang, Xin.

[12]De novo transcriptome sequencing of black pepper (Piper nigrum L.) and an analysis of genes involved in phenylpropanoid metabolism in response to Phytophthora capsici. Hao, Chaoyun,Fan, Rui,Tan, Lehe,Hu, Lisong,Wu, Baoduo,Wu, Huasong,Xia, Zhiqiang,Hao, Chaoyun,Tan, Lehe,Hu, Lisong,Wu, Huasong,Fan, Rui,Wu, Baoduo,Wu, Huasong. 2016

[13]Efficient de novo synthesis of resveratrol by metabolically engineered Escherichia coli. Wu, Junjun,Zhou, Peng,Zhang, Xia,Dong, Mingsheng,Wu, Junjun,Zhou, Peng,Zhang, Xia,Dong, Mingsheng.

[14]Stepwise modular pathway engineering of Escherichia coli for efficient one-step production of (2S)-pinocembrin. Wu, Junjun,Zhang, Xia,Dong, Mingsheng,Wu, Junjun,Zhou, Jingwen,Wu, Junjun,Zhang, Xia,Dong, Mingsheng.

[15]Relationships between endogenous hormonal content and direct somatic embryogenesis in Watermelon (Citrullus lanatus) cotyledons. Zhang, Hui Jun,Wang, Qing,Wang, Qing. 2015

[16]Abiotic Stresses and Phytohormones Regulate Expression of FAD2 Gene in Arabidopsis thaliana. Yuan Si-wei,Wu Xue-long,Liu Zhi-hong,Huang Rui-zhi,Yuan Si-wei,Luo Hong-bing. 2012

[17]Regulatory mechanisms of oxidative species and phytohormones in marine microalgae Isochrysis zhangjiangensis under nitrogen deficiency. Wu, Shuang,Meng, Yingying,Cao, Xupeng,Xue, Song,Wu, Shuang. 2016

[18]Simultaneous determination of 13 phytohormones in oilseed rape tissues by liquid chromatography-electrospray tandem mass spectrometry and the evaluation of the matrix effect. Fan, Sufang,Wang, Xiupin,Li, Peiwu,Zhang, Qi,Zhang, Wen,Fan, Sufang,Wang, Xiupin,Li, Peiwu,Zhang, Qi,Zhang, Wen,Fan, Sufang,Wang, Xiupin,Li, Peiwu.

[19]Carbon nanomaterials alter plant physiology and soil bacterial community composition in a rice-soil-bacterial ecosystem. Hao, Yi,Zhang, Zetian,Rui, Yukui,Liu, Liming,Ma, Chuanxin,Rui, Yukui,Xing, Baoshan,Ma, Chuanxin,Song, Youhong,Cao, Weidong,Zhou, Guopeng,Guo, Jing. 2018

[20]Further insight into the role of KAN1, a member of KANADI transcription factor family in rice. Adedze, Yawo Mawunyo Nevame,Feng, Baobing,Shi, Lei,Sheng, Zhonghua,Tang, Shaoqing,Wei, Xiangjin,Hu, Peisong. 2018

作者其他论文 更多>>

微信小程序