Porcine epidemic diarrhea virus nucleoprotein contributes to HMGB1 transcription and release by interacting with C/EBP-beta

文献类型: 外文期刊

第一作者: Huan, Chang-chao

作者: Huan, Chang-chao;Wang, Hua-xia;Sheng, Xiang-xiang;Wang, Rui;Wang, Xin;Fan, Hong-jie;Mao, Xiang;Liao, Ying;Liu, Qin-fang;Tong, Guang-zhi;Ding, Chan;Mao, Xiang;Wu, Jia-qiang

作者机构:

关键词: porcine epidemic diarrhea virus;HMGB1;nucleoprotein;C/EBP-beta

期刊名称:ONCOTARGET ( 影响因子:5.168; 五年影响因子:5.312 )

ISSN: 1949-2553

年卷期: 2016 年 7 卷 46 期

页码:

收录情况: SCI

摘要: Porcine epidemic diarrhea is a devastating swine enteric disease, which is caused by porcine epidemic diarrhea virus (PEDV) infection. Our studies demonstrated that PEDV infection resulted in the up-regulation of proinflammatory cytokines. Meanwhile, PEDV infection and overexpression of viral nucleoprotein resulted in the acetylation and release of high mobility group box 1 proteins in vitro, an important proinflammatory response mediator, which contributes to the pathogenesis of various inflammatory diseases. Our studies also showed that SIRT1, histone acetyltransferase, and NF-kappa B regulated the acetylation and release of HMGB1. Chromatin immunoprecipitation, dual-luciferase reporter gene assay, and co-immunoprecipitation experiments illustrated that PEDV-N could induce HMGB1 transcription by interacting with C/EBP-beta, which could bind to C/EBP motif in HMGB1 promotor region. Collectively, our data indicate PEDV-N contributes to HMGB1 transcription and the subsequent release/acetylation of HMGB1 during PEDV infection.

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