Melatonin improves reprogramming efficiency and proliferation of bovine-induced pluripotent stem cells

文献类型： 外文期刊

第一作者： Bai, Chunyu

作者： Bai, Chunyu;Li, Xiangchen;Gao, Yuhua;Yuan, Ziao;Hu, Pengfei;Wang, Hui;Liu, Changqing;Guan, Weijun;Ma, Yuehui;Gao, Yuhua;Yuan, Ziao;Hu, Pengfei;Wang, Hui;Liu, Changqing

作者机构：

关键词： cell proliferation;cell reprogramming;induced pluripotent stem cells;melatonin;neural stem cells

期刊名称：JOURNAL OF PINEAL RESEARCH （ 影响因子：13.007； 五年影响因子：12.936 ）

ISSN： 0742-3098

年卷期： 2016 年 61 卷 2 期

页码：

收录情况： SCI

摘要： Melatonin can modulate neural stem cell (NSC) functions such as proliferation and differentiation into NSC-derived pluripotent stem cells (N-iPS) in brain tissue, but the effect and mechanism underlying this are unclear. Thus, we studied how primary cultured bovine NSCs isolated from the retinal neural layer could transform into N-iPS cell. NSCs were exposed to 0.01, 0.1, 1, 10, or 100 m melatonin, and cell viability studies indicated that 10 m melatonin can significantly increase cell viability and promote cell proliferation in NSCs in vitro. Thus, 10 m melatonin was used to study miR-302/367-mediated cell reprogramming of NSCs. We noted that this concentration of melatonin increased reprogramming efficiency of N-iPS cell generation from primary cultured bovine NSCs and that this was mediated by downregulation of apoptosis-related genes p53 and p21. Then, N-iPS cells were treated with 1, 10, 100, or 500 m melatonin, and N-iPS (M-N-iPS) cell proliferation was measured. We noted that 100 m melatonin increased proliferation of N-iPS cells via increased phosphorylation of intracellular ERK1/2 via activation of its pathway in M-N-iPS via melatonin receptors 1 (MT1). Finally, we verified that N-iPS cells and M-N-iPS cells are similar to typical embryonic stem cells including the expression of pluripotency markers (Oct4 and Nanog), the ability to form teratomas in vivo, and the capacity to differentiate into all three embryonic germ layers.

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