Development of cross-priming amplification coupled with vertical flow visualization for rapid detection of infectious spleen and kidney necrosis virus (ISKNV) in mandarin fish, Siniperca chuatsi

文献类型： 外文期刊

第一作者： Liu, Wenzhi

作者： Liu, Wenzhi;Zhou, Yong;Fan, Yuding;Jiang, Nan;Zeng, Lingbing;Cain, Kenneth;Cain, Kenneth

作者机构：

关键词： Mandarin fish (Siniperca chuatsi);Infectious spleen and kidney necrosis virus (ISKNV);Cross-priming amplification coupled with vertical flow visualization (CPA-VF);Detection

期刊名称：JOURNAL OF VIROLOGICAL METHODS （ 影响因子：2.014； 五年影响因子：2.001 ）

ISSN： 0166-0934

年卷期： 2018 年 253 卷

页码：

收录情况： SCI

摘要： Infectious spleen and kidney necrosis virus (ISKNV) has been recognized as the causative agent of the most serious disease in cultured mandarin fish, Siniperca chuatsi, in China. Disease outbreaks have resulted in substantial losses to the aquaculture industry. Currently, reliable laboratory detection and identification methods are available for this virus. However, rapid detection methods applicable for on-site diagnosis of this infectious agent are unavailable. To address this need, a nearly instrument-free, cost-effective and simple detection method was developed and optimized and incorporates cross priming amplification coupled with vertical flow visualization for rapid identification of ISKNV (ISKNV-CPA-VF). Results show that cross circulation amplification targeting the conserved region of the major capsid protein (MCP) regiment of the ISKNV genome had a sensitivity 10 times greater than traditional PCR at 64 degrees C within 60 min. The optimized concentration of dNTPs and the concentration for Mg2+ were 1.0 mmol/L and 10 mmol/L, respectively. No cross-reactions with other viruses or bacteria were observed. When combined with the nucleic acid strip detection technology, visual detection of ISKNV amplified products was realized within 3-5 min following amplification. The simplicity and nearly instrument-free method for this ISKNV-CPA-VF assay shows great potential for on-site diagnostics of ISKNV infection in Siniperca chuatsi.

分类号：

  • 相关文献

[1]Establishment and application of cross-priming isothermal amplification coupled with lateral flow dipstick (CPA-LFD) for rapid and specific detection of red-spotted grouper nervous necrosis virus. Su, Zi Dan,Shi, Cheng Yin,Huang, Jie,Shen, Gui Ming,Li, Jin,Wang, Sheng Qiang,Fan, Chao,Shi, Cheng Yin,Huang, Jie,Su, Zi Dan,Shen, Gui Ming,Wang, Sheng Qiang,Fan, Chao. 2015

[2]Detection and quantification of hepatopancreatic parvovirus in penaeid shrimp by real-time PCR assay. Liu, Tianqi,Yang, Bing,Song, Xiaoling,Wang, Xiuhua,Yuan, Yanyan,Liu, Li,Huang, Jie,Liu, Tianqi,Yuan, Yanyan. 2013

[3]Development of a real-time reverse transcription loop-mediated isothermal amplification method for the rapid detection of porcine epidemic diarrhea virus. Yu, Xuewu,Shi, Lin,Lv, Xiaoping,Zheng, Shimin,Yu, Xuewu,Shi, Lin,Yao, Wei,Cao, Minghui,Yu, Hanxun,Wang, Xiurong,Wang, Xiurong. 2015

[4]An improved method for detection of Edwardsiella tarda by loop-mediated isothermal amplification by targeting the EsrB gene. Xie Guosi,Zhang Qingli,Han Nana,Shi Chengyin,Wang Xiuhua,Liu Qinghui,Huang Jie,Xie Guosi,Huang Jie. 2012

[5]Rapid detection of porcine parvovirus DNA by sensitive loop-mediated isothermal amplification. Chen, Hao-tai,Zhang, Jie,Yang, Sheng-hai,Ma, Li-na,Ma, Yan-ping,Liu, Xiang-tao,Cai, Xue-peng,Zhang, Yong-guang,Liu, Yong-sheng. 2009

[6]Rapid detection of porcine circovirus type 2 by loop-mediated isothermal amplification. Chen, Hao-tai,Zhang, Jie,Sun, De-hui,Chu, Yue-feng,Cai, Xue-peng,Liu, Xiang-tao,Luo, Xue-nong,Liu, Yong-sheng,Liu, Qing. 2008

[7]Development and validation of a TaqMan (TM) fluorescent quantitative real-time PCR assay for the rapid detection of Edwardsiella tarda. Xie Guosi,Huang Jie,Zhang Qingli,Han Nana,Shi Chengyin,Wang Xiuhua,Xie Guosi,Huang Jie. 2012

[8]Quantitative detection of the rice false smut pathogen Ustilaginoidea virens by real-time PCR. Li, H.,Ni, D. H.,Li, J.,Li, H.,Ni, D. H.,Duan, Y. B.,Li, J.,Song, F. S.,Li, L.,Wei, P. C.,Yang, J. B.,Li, H.,Wei, P. C.,Duan, Y. B.,Song, F. S.,Chen, Y.. 2013

[9]Rapid typing of foot-and-mouth disease serotype Asia 1 by reverse transcription loop-mediated isothermal amplification. Liu, Yong-sheng,Liu, Xiang-tao. 2011

[10]The Applications of Gold Nanoparticle-Initialed Chemiluminescence in Biomedical Detection. Liu, Zezhong,Zhao, Furong,Gao, Shandian,Shao, Junjun,Chang, Huiyun. 2016

[11]A Miniature Handheld Automatic Detected Device for Register. Zhang Tiefeng,Wang Jun,Sun Zhihui,Zhao Bo,Lu Guotao. 2011

[12]DEVELOPMENT OF A SIMPLE AND EFFECTIVE METHOD FOR SPECIFIC DETECTION OF PEPPER MILD MOTTLE VIRUS. Liu, Y.,Wang, X.,Zhou, G.,Li, X.. 2009

[13]Aptamer-Based Fluorescence Assay for Hg2+ Determination. Lei Zhao-Jing,Hu Qiu-Hui,Lei Zhao-Jing,Zhang Cun-Zheng,Liu Yuan,Zhang Qiang,Liu Xian-Jin. 2012

[14]Detection and quantification of Rhizoctonia cerealis in soil using real-time PCR. Guo, Yingpeng,Li, Wei,Sun, Haiyan,Wang, Ning,Chen, Huaigu,Guo, Yingpeng,Yu, Hanshou. 2012

[15]A real-time PCR targeted to the upstream regions of HlyB for specific detection of Edwardsiella tarda. Xie Guosi,Huang Jie,Zhang Qingli,Han Nana,Shi Chengyin,Wang Xiuhua,Liu Qinghui,Xie Guosi,Huang Jie. 2012

[16]Development of a loop-mediated isothermal amplification assay for rapid and sensitive detection of ostreid herpesvirus 1 DNA. Ren, Weicheng,Cai, Yuyong,Wang, Chongming,Renault, Tristan,Cai, Yuyong. 2010

[17]Fluorescence polarization assay for the simultaneous determination of bisphenol A, bisphenol F and their diglycidyl ethers in canned tuna. Guan, Tianzhu,Li, Tiezhu,Li, Zhuolin,Wang, Yongzhi,Zhang, Jie,Wang, Yongjun,Zhang, Tiehua,Yu, Hansong,Ruan, Ping. 2017

[18]Validation of high-throughput real time polymerase chain reaction assays for simultaneous detection of invasive citrus pathogens. Loconsole, Giuliana,Savino, Vito,Liao, Hui-Hong,Jiang, Bo,Saponari, Maria,Yokomi, Raymond K.. 2013

[19]Rapid detection of Haemophilus parasuis using cross-priming amplification and vertical flow visualization. Li, Chunling. 2018

[20]Development of a loop-mediated isothermal amplification method for rapid detection of porcine boca-like virus. Li, Bin,Ma, Jun-jie,Zhang, Xue-han,Wen, Li-bin,Mao, Li,Ni, Yan-xiu,Guo, Rong-li,Zhou, Jun-ming,Lv, Li-xin,He, Kong-wang,Li, Bin,Ma, Jun-jie,Zhang, Xue-han,Wen, Li-bin,Mao, Li,Ni, Yan-xiu,Guo, Rong-li,Zhou, Jun-ming,Lv, Li-xin,He, Kong-wang,Li, Bin,Ma, Jun-jie,Zhang, Xue-han,Wen, Li-bin,Mao, Li,Ni, Yan-xiu,Guo, Rong-li,Zhou, Jun-ming,Lv, Li-xin,He, Kong-wang,Xiao, Shao-bo. 2012

作者其他论文 更多>>

微信小程序