Newly identified invertebrate-type lysozyme (Splys-i) in mud crab (Scylla paramamosain) exhibiting muramidase-deficient antimicrobial activity

文献类型： 外文期刊

第一作者： Zhou, Jian

作者： Zhou, Jian;Zhao, Shu;Fang, Wen-Hong;Zhou, Jun-Fang;Zhang, Jing-Xiao;Li, Xin-Cang;Zhou, Jian;Zhao, Shu;Fang, Wen-Hong;Zhou, Jun-Fang;Zhang, Jing-Xiao;Li, Xin-Cang;Zhou, Jian;Ma, Hongyu;Lan, Jiang-Feng

作者机构：

关键词： Invertebrate-type lysozyme;Expression profiles;Antimicrobial activity;Binding activity;Agglutinating activity;Isopeptidase activity

期刊名称：DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY （ 影响因子：3.636； 五年影响因子：3.654 ）

ISSN： 0145-305X

年卷期： 2017 年 74 卷

页码：

收录情况： SCI

摘要： Lysozymes are widely distributed immune effectors exerting muramidase activity against the peptidoglycan of the bacterial cell wall to trigger cell lysis. However, some invertebrate-type (i-type) lysozymes deficient of muramidase activity still exhibit antimicrobial activity. To date, the mechanism underlying the antimicrobial effect of muramidase-deficient i-type lysozymes remains unclear. Accordingly, this study characterized a novel i-type lysozyme, Splys-i, in the mud crab Scylla paramamosain. Splys-i shared the highest identity with the Litopenaeus vannamei i-type lysozyme (Lvlys-i2, 54% identity) at the amino acid level. Alignment analysis and 3D structure comparison show that Splys-i may be a muramidasedeficient i-type lysozyme because it lacks the two conserved catalytic residues (Glu and Asp) that are necessary for muramidase activity. Splys-i is mainly distributed in the intestine, stomach, gills, hepatopancreas, and hemocytes, and it is upregulated by Vibrio harveyi or Staphylococcus aureus challenge. Recombinant Splys-i protein (rSplys-i) can inhibit the growth of Gram-negative bacteria (V harveyi, Vibrio alginolyticus, Vibrio parahemolyticus, and Escherichia coli), Gram-positive bacteria (S. aureus, Bacillus subtilis, and Bacillus megaterium), and the fungus Candida albicans to varying degrees. In this study, two binding assays and a bacterial agglutination assay were conducted to elucidate the potential antimicrobial mechanisms of Splys-i. Results demonstrated that rSplys-i could bind to all nine aforementioned microorganisms. It also exhibited a strong binding activity to lipopolysaccharide from E. coli and lipoteichoic acid and peptidoglycan (PGN) from S. aureus but a weak binding activity to PGN from B. subtilis and beta-glucan from fungi. Moreover, rSplys-i could agglutinate these nine types of microorganisms in the presence of Ca2+ at different protein concentrations. These results suggest that the binding activity and its triggered agglutinating activity might be two major mechanisms of action to realize the muramidasedeficient antibacterial activity. In addition, rSplys-i can hydrolyze the peptidoglycan of some Grampositive bacteria because it exhibits weak isopeptidase activities in salt and protein concentration dependent manner. This result indicates that such an isopeptidase activity may contribute to the muramidase-deficient antimicrobial activity to a certain degree. In conclusion, Splys-i is upregulated by pathogenic bacteria, and it inhibits bacterial growth by binding and agglutination activities as well as isopeptidase activity, suggesting that Splys-i is involved in immune defense against bacteria through several different mechanisms of action. (C) 2017 Elsevier Ltd. All rights reserved.

分类号：

  • 相关文献

[1]SpALF4: A newly identified anti-lipopolysaccharide factor from the mud crab Scylla paramamosain with broad spectrum antimicrobial activity. Zhu, Lei,Huang, Yan-Qing,Zhou, Jun-Fang,Fang, Wen-Hong,Yao, Xiao-Juan,Wang, Hao,Li, Xin-Cang,Zhu, Lei,Huang, Yan-Qing,Zhou, Jun-Fang,Fang, Wen-Hong,Yao, Xiao-Juan,Wang, Hao,Li, Xin-Cang,Lan, Jiang-Feng,Zhang, Chao. 2014

[2]Test of hirudin activity by tracking the binding of hirudin to thrombin in the presence of BS3 cross-linking. Liu, Yanfang,Wang, Jiangmin,Huang, Qingmei,Yang, Xiaohong,Zhang, Jianhua,Yang, Jian. 2015

[3]Immunolocation and enzyme activity analysis of Cryptosporidium parvum enolase. Mi, Rongsheng,Chen, Zhaoguo,Mi, Rongsheng,Yang, Xiaojiao,Huang, Yan,Cheng, Long,Lu, Ke,Han, Xiangan,Chen, Zhaoguo,Mi, Rongsheng,Yang, Xiaojiao,Huang, Yan,Cheng, Long,Lu, Ke,Han, Xiangan,Chen, Zhaoguo. 2017

[4]CkDREB gene in Caragana korshinskii is involved in the regulation of stress response to multiple abiotic stresses as an AP2/EREBP transcription factor. Wang, Xuemin,Chen, Xiaofang,Gao, Hongwen,Wang, Zan,Sun, Guizhi,Liu, Yun.

[5]Chiral beta-arylalkyl-1H-1,2,4-triazoles as demethylase inhibitors: Biological evaluation and its stereoselective interaction with sterol 14 alpha-demethylase from Penicillium digitatum. Cao, Xiufang,Chen, Changshui,Ke, Shaoyong.

[6]A novel myeloid differentiation factor 88 homolog, SpMyD88, exhibiting SpToll-binding activity in the mud crab Scylla paramamosain. Li, Xin-Cang,Zhu, Lei,Li, Lin-Gui,Huang, Yan-Qing,Lu, Jian-Xue,Fang, Wen-Hong,Kang, Wei,Ren, Qian,Ren, Qian.

[7]Expression profiles of miRNAs in Gossypium raimondii. Jun MA,Teng-long GUO,Qing-lian WANG,Kun-bo WANG,Run-run SUN,Bao-hong ZHANG. 2015

[8]Proline accumulation and metabolism-related genes expression profiles in Kosteletzkya virginica seedlings under salt stress. Wang, Hongyan,Tang, Xiaoli,Shao, Hong-Bo,Wang, Hongyan,Wang, Honglei,Wang, Hongyan,Tang, Xiaoli,Shao, Hong-Bo. 2015

[9]The Mouthparts Enriched Odorant Binding Protein 11 of the Alfalfa Plant Bug Adelphocoris lineolatus Displays a Preferential Binding Behavior to Host Plant Secondary Metabolites. Xiao, Qiang,Sun, Liang,Wei, Yu,Ma, Xiao-Yu,Xiao, Yong,Yang, Xian-Ming,Guo, Yu-Yuan,Zhang, Yong-Jun,Zhang, Dan-Dan,Zhang, Ya-Nan. 2016

[10]Identification of the genes in tea leafhopper, Empoasca onukii (Hemiptera: Cicadellidae), that encode odorant-binding proteins and chemosensory proteins using transcriptome analyses of insect heads. Bian, Lei,Li, Zhao-Qun,Cai, Xiao-Ming,Luo, Zong-Xiu,Chen, Zong-Mao,Ma, Long. 2018

[11]Genetic Regulation of GA Metabolism during Vernalization, Floral Bud Initiation and Development in Pak Choi (Brassica rapa ssp chinensis Makino). Shang, Mengya,Wang, Xueting,Zhang, Jing,Ping, Amin,Hou, Leiping,Xing, Guoming,Li, Meilan,Qi, Xianhui,Li, Gaizhen. 2017

[12]Expression profiles of the p38 MAPK signaling pathway from Chinese shrimp I Fenneropenaeus chinensis in response to viral and bacterial infections. He, Yuying,Liu, Ping,Li, Jian,Wang, Qingyin,He, Yuying,Li, Jian,Yao, Wanlong. 2018

[13]Characterization and functional analysis of a chitin synthase gene (HcCS1) identified from the freshwater pearlmussel Hyriopsis cumingii. Zheng, H. F.,Bai, Z. Y.,Lin, J. Y.,Wang, G. L.,Li, J. L.,Zheng, H. F.,Li, J. L.. 2015

[14]Dynamic Expression of MicroRNA-127 During Porcine Prenatal and Postnatal Skeletal Muscle Development. Yang Ya-lan,Li Yan,Liang Ru-yi,Zhou Rong,Ao Hong,Mu Yu-lian,Yang Shu-lin,Li Kui,Tang Zhong-lin,Li Yan. 2014

[15]Characterization of MUSTN1 gene and its relationship with skeletal muscle development at postnatal stages in Pekin ducks. Xu, T. S.,Gu, L. H.,Liu, X. L.,Xu, T. S.,Xu, T. S.,Hou, S. S.,Zhang, X. H.,Sun, Y.,Ye, B. G.. 2015

[16]Identification and expression profile analysis of odorant-binding protein genes in Apolygus lucorum (Hemiptera: Miridae). Gu, Shao-Hua,Liu, Jing-Tao,Zhu, Xiao-Qiang,Guo, Yu-Yuan,Zhang, Yong-Jun,Liu, Jing-Tao,Zhou, Jing-Jiang.

[17]Molecular cloning, expression profiles, and characterization of a novel polyphenol oxidase (PPO) gene in Hevea brasiliensis. Li, Dejun,Deng, Zhi,Liu, Changren,Zhao, Manman,Guo, Huina,Liu, Hui,Liu, Changren,Guo, Huina,Xia, Zhihui. 2014

[18]Genome-wide identification and expression analysis of the BTB domain-containing protein gene family in tomato. Li, Jinhua,Su, Xiaoxing,Yang, Wei,Pan, Yu,Su, Chenggang,Zhang, Xingguo,Li, Jinhua,Su, Xiaoxing,Yang, Wei,Pan, Yu,Su, Chenggang,Zhang, Xingguo,Wang, Yinlei. 2018

[19]cDNA structure, genomic organization and expression patterns of visfatin in silver Prussian carp (Carassius auratus gibelio). Dong Xu,Zou Shiping,Wu Xiaoxiong,Dai Hanchuan,Zeng Cuiping,Wang Xu,Long Liangqi,Zou Shiping. 2011

[20]Identification and Characterization of Odorant Binding Proteins in the Forelegs of Adelphocoris lineolatus (Goeze). Sun, Liang,Wang, Qian,Wang, Qi,Dong, Kun,Xiao, Yong,Zhang, Yong-Jun,Sun, Liang,Wang, Qian. 2017

作者其他论文 更多>>

微信小程序