V2 Protein Enhances the Replication of Genomic DNA of Mulberry Crinkle Leaf Virus

文献类型： 外文期刊

第一作者： Yin, Zhen-Ni

作者： Yin, Zhen-Ni;Han, Pei-Yu;Han, Tao-Tao;Huang, Ying;Yang, Jing-Jing;Zhang, Meng-Si;Fang, Miao;Zhong, Kui;Zhang, Jian;Lu, Quan-You;Fang, Miao;Zhong, Kui;Zhang, Jian;Lu, Quan-You

作者机构：

关键词： geminivirus; mulberry crinkle leaf virus; V2; replication enhancer

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：4.9； 五年影响因子：5.7 ）

ISSN： 1661-6596

年卷期： 2024 年 25 卷 19 期

页码：

收录情况： SCI

摘要： Mulberry crinkle leaf virus (MCLV), identified in mulberry plants (Morus alba L.), is a member of the genus Mulcrilevirus in the family Geminiviridae. The functions of the V2 protein encoded by MCLV remain unclear. Here, Agrobacterium-mediated infectious clones of a wild-type MCLV vII (MCLVWT) and two V2 mutant MCLV vIIs, including MCLVmV2 (with a mutation of the start codon of the V2 ORF) and MCLVdV2 (5 '-end partial deletion of the V2 ORF sequence), were constructed to investigate the roles of V2 both in planta and at the cellular level. Although all three constructs (pCA-1.1MCLVWT, pCA-MCLVmV2, and pCA-MCLVdV2) were able to infect both natural host mulberry plants and experimental tomato plants systematically, the replication of the MCLVmV2 and MCLVdV2 genomes in these hosts was significantly reduced compared to that of MCLVWT. Similarly, the accumulation of MCLVmV2 and MCLVdV2 in protoplasts of Nicotiana benthamiana plants was significantly lower than that of MCLVWT either 24 h or 48 h post-transfection. A complementation experiment further confirmed that the decreased accumulation of MCLV in the protoplasts was due to the absence of V2 expression. These results revealed that MCLV-encoded V2 greatly enhances the level of MCLV DNA accumulation and is designated the replication enhancer protein of MCLV.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序