Cloning of catalase and expression patterns of catalase and selenium-dependent glutathione peroxidase from Exopalaemon carinicauda in response to low salinity stress

文献类型： 外文期刊

第一作者： Ren Hai

作者： Ren Hai;Ren Hai;Li Jian;Li Jitao;Ying Yu;Ge Hongxing;Li Dongli;Yu Tianji

作者机构：

关键词： Exopalaemon carinicauda;catalase (CAT);selenium-dependent glutathione peroxidase (Se-GPx);cloning;expression

期刊名称：ACTA OCEANOLOGICA SINICA （ 影响因子：1.431； 五年影响因子：1.445 ）

ISSN： 0253-505X

年卷期： 2015 年 34 卷 8 期

页码：

收录情况： SCI

摘要： Catalase (CAT) and selenium-dependent glutathione peroxidase (Se-GPx) play a vital role in protecting organisms against various oxidative stresses by eliminating H2O2. The objective of this paper is to evaluate the roles of these antioxidant molecules in the ridgetail white prawn Exopalaemon carinicauda in response to low salinity stress. A complementary DNA (cDNA) containing the complete coding sequence of CAT was cloned from the hepatopancreas using reverse-transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends. The full-length cDNA of CAT (2 649 bp) contains a 5'-untranslated region (UTR) of 78 bp, a 3'-UTR of 1 017 bp, with a poly (A) tail, and an open reading frame of 1 554 bp encoding a 517-amino-acid polypeptide with predicted molecular mass of 58.46 kDa and estimated isoelectric point of 6.64. This CAT sequence contained the proximal active site signature ((60)FDRERIPERVVHAKGAG(76)), proximal heme-ligand signature sequence ((RLFSYPDTH358)-R-350) and three catalytic amino acid residues (His(71), Asn(144) and Tyr(354)). Sequence comparison showed that the CAT deduced amino acid sequence of E. carinicauda shared 68%-92% of identities with those of other species. Quantitative real-time PCR analysis revealed that CAT mRNA was widely expressed in the hepatopancreas (highest), hemocyte, eyestalk, heart, gill, muscle, ovary and stomach. Under low salinity stress, CAT and GPx mRNA expression levels both in the gill and hepatopancreas increased significantly at the first 48 h and 6 h respectively, indicating a tissue- and time-dependent antioxidant response in E. carinicauda. All these results indicate that E. carinicauda CAT is a member of the CAT family and might be involved in the acute response against low salinity stress.

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