Separation, Purification, and Identification of (3S,3 ' S)-trans-Astaxanthin from Haematococcus pluvialis

文献类型： 外文期刊

第一作者： Sun, Weihong

作者： Sun, Weihong;Lin, Hong;Cao, Limin;Sun, Weihong;Zhai, Yuxiu;Leng, Kailiang;Xing, Lihong

作者机构：

关键词： identification;Haematococcus pluvialis;separation;(3S,3 ' S)-trans-astaxanthin;purification

期刊名称：SEPARATION SCIENCE AND TECHNOLOGY （ 影响因子：2.475； 五年影响因子：2.077 ）

ISSN： 0149-6395

年卷期： 2015 年 50 卷 9 期

页码：

收录情况： SCI

摘要： A simple, low-cost method of separation and purification for preparing (3S,3 ' S)-trans-astaxanthin from Haematococcus pluvialis was established in this study. Crude extracts were separated from dry algal cells by extraction. The extracts were then saponified at 4 degrees C for 15 h and at 22 degrees C for 3 h with 0.02 M NaOH in the reaction mixture with lower isomerization of trans-astaxanthin to cis isomers. The saponified pigment extracts were mixed with distilled water and n-hexane at 1:1:1 volume ratio. Low-pressure column chromatography of silica gel (300-400 mesh) with different eluents was used to prepare high-purity (> 80%) all-trans-astaxanthin. Supersaturated solution of astaxanthin in acetone was then crystallized for 72 h at 4 degrees C, and the purity of all-trans-astaxanthin crystalline powder was confirmed to be > 96.5% by HPLC. And the 3S,3 ' S isomers were identified and characterized by HPLC-APCI-MS, H-1 NMR, C-13 NMR, and HPLC-UV.

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