Cytoplasmic dynein1 intermediate-chain2 regulates cellular trafficking and physiopathological development in Magnaporthe oryzae

文献类型： 外文期刊

第一作者： Lin, Lili

作者： Lin, Lili;Tijjani, Ibrahim;An, Qiuli;Cao, Jiaying;Chen, Xiaomin;Wang, Zonghua;Guo, Hengyuan;Norvienyeku, Justice;Lin, Lili;Liu, Wende;Norvienyeku, Justice;Wang, Zonghua

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期刊名称：ISCIENCE （ 影响因子：5.8； 五年影响因子：5.8 ）

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年卷期： 2023 年 26 卷 2 期

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收录情况： SCI

摘要： The cytoplasmic dynein 1, a minus end-directed motor protein, is an essential microtubule-based molecular motor that mediates the movement of molecules to intracellular destinations in eukaryotes. However, the role of dynein in the pathogenesis of Magnaporthe oryzae is unknown. Here, we identified cyto-plasmic dynein 1 intermediate-chain 2 genes in M. oryzae and functionally charac-terized it using genetic manipulations, and biochemical approaches. We observed that targeted the deletion of MoDYNC1I2 caused significant vegetative growth defects, abolished conidiation, and rendered the Delta Modync1I2 strains non-pathogenic. Microscopic examinations revealed significant defects in microtubule network organization, nuclear positioning, and endocytosis Delta Modync1I2 strains. MoDync1I2 is localized exclusively to microtubules during fungal developmental stages but co-localizes with the histone OsHis1 in plant nuclei upon infection. The exogenous expression of a histone gene, MoHis1, restored the homeostatic phenotypes of Delta Modync1I2 strains but not pathogenicity. These findings could facil-itate the development of dynein-directed remedies for managing the rice blast disease.

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