Antibacterial mechanism of lysine against Escherichia coli O157:H7, its action on lettuce and in reducing the severity of murine colitis

文献类型： 外文期刊

第一作者： Tian, Lei

作者： Tian, Lei;Ma, Luyao;Tian, Yudong;Zhang, Chunling;Wang, Xin;Zhou, Ting;Pan, Hu;Dai, Yanna;Pu, Jifeng;Fu, Chengyu;Zhang, Pengfei

作者机构：

关键词： Lysine; Escherichia coli O157:H7; Antibacterial mechanism; Lettuce; Colitis

期刊名称：FOOD MICROBIOLOGY （ 影响因子：4.6； 五年影响因子：5.1 ）

ISSN： 0740-0020

年卷期： 2025 年 129 卷

页码：

收录情况： SCI

摘要： The aim of the present study was to evaluate the mechanism of antimicrobial action of Lysine (Lys) against Escherichia coli (E. coli) O157:H7. Lys alters the permeability and morphology of bacterial cell membranes, leading to leakage of intracellular material. Using agarose gel electrophoresis and sodium dodecyl sulfatepolyacrylamide gel electrophoresis(SDS-PAGE), it was found that high concentrations of Lys disrupted the DNA and protein primary structure of E. coli O157:H7. Lys also inhibited the ability of E. coli biofilm formation in a concentration-dependent manner. Subinhibitory concentration of Lys reduces the swimming and swarming ability of E. coli O157:H7 and its ability to adhere and to invade Caco-2 cells. The use of Lys on lettuce inoculated with E. coli O157:H7 showed good disinfection efficiency. Further studies revealed that Lys had both preventive and therapeutic effects on the severity of colitis induced by E. coli O157:H7 infected mice, and that the preventive effect was greater than the therapeutic effect.

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