Functional Characterization of CsBAS1, CsSND1, and CsIRX6 in Cucumber Defense Against Meloidogyne incognita

文献类型： 外文期刊

第一作者： Li, Shihui

作者： Li, Shihui;Wang, Xueyun;Gao, Lihong;Tian, Yongqiang;Ma, Si;Ma, Si

作者机构：

关键词： cucumber; vascular bundle; Meloidogyne incognita; giant cell

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：4.9； 五年影响因子：5.7 ）

ISSN： 1661-6596

年卷期： 2025 年 26 卷 5 期

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收录情况： SCI

摘要： Vascular tissue development plays a pivotal role in plant growth and defense against biotic stress. Root-knot nematodes, particularly Meloidogyne incognita (M. incognita), are globally distributed phytopathogens that cause severe economic losses in a variety of vascular plants. In this study, three vascular bundle development-related genes, including CsBAS1, CsSND1, and CsIRX6, were identified in cucumber. Tissue-specific expression analysis revealed that CsSND1 and CsIRX6 were highly expressed in roots. Infection with M. incognita showed dynamic expression changes for CsBAS1, CsSND1, and CsIRX6. Specially, CsIRX6 and CsSND1 were upregulated at 14 days post-inoculation (dpi), while CsBAS1 was downregulated at both 7 dpi and 14 dpi. Tissue localization studies using promoter-GUS constructs demonstrated pCsBAS1-GUS and pCsSND1-GUS activity in galls and specific vascular tissues, while CsIRX6 mRNA was detected in giant cells (GCs) at 14 dpi using in situ methods. Virus-induced gene silencing (VIGS) of CsBAS1, CsSND1, and CsIRX6 revealed their distinct roles in nematode-induced gall formation. Silencing CsBAS1 and CsSND1 resulted in increased root growth and gall size, whereas silencing CsIRX6 led to reduced gall size. These findings highlight the functional significance of CsBAS1, CsSND1, and CsIRX6 in cucumber defense against M. incognita, offering insights into the interplay between vascular development and plant defense mechanisms.

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