In vivo imaging via a red-emitting fluorescent probe to diagnosing liver cancer or drug-induced liver disease

文献类型： 外文期刊

第一作者： Yang, Yuexia

作者： Yang, Yuexia;Wang, Jiemin;Cao, Ting;Zheng, Lei;Cao, Yuping;Qin, Wenwu;Yang, Yuexia;Wang, Jiemin;Cao, Ting;Zheng, Lei;Cao, Yuping;Qin, Wenwu;Zhang, Liang;Liu, Yun;Li, Jiajun;Li, Jiajun

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关键词： Liver injury; Red-emitting fluorescent probe; Leucine aminopeptidase; Mice imaging; In vivo and vitro

期刊名称：ANALYTICA CHIMICA ACTA （ 影响因子：6.558； 五年影响因子：6.228 ）

ISSN： 0003-2670

年卷期： 2021 年 1168 卷

页码：

收录情况： SCI

摘要： Leucine aminopeptidase (LAP) as an important proteolytic enzyme, has been mainly found in hepatobiliary cells, and overexpressed in hepatoma cells. Herein, a new highly selective red-emitting fluorescent probe (DCDHF-Ala) for LAP has been synthesized based on 2-dicyanomethyldiene-3-cyano-2, 5-dihydrofuran (DCDHF) as fluorophore, and alanine (Ala) as the detection group. More importantly, it's the first time to use Ala as a reactive group for LAP. DCDHF-Ala has a low detection limit (0.20 U/L), excellent water solubility and cell membrane permeability. In addition, the probe has been successfully applied to fluorescent imaging in cells and zebrafish. It's especially worth mentioning that, DCDHF-Ala has a high biosafety and enables a real-time detection of LAP levels in mice model. What's the most important is that DCDHF-Ala may be an effective tool to qualitatively monitor the upregulation of LAP induced by liver injury and liver cancer. (C) 2021 Elsevier B.V. All rights reserved.

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