Dysfunction of GmVPS8a causes compact plant architecture in soybean

文献类型： 外文期刊

第一作者： Kong, Keke

作者： Kong, Keke;Xu, Mengge;Xu, Zhiyong;Lv, Wenhuan;Lv, Peiyun;Begum, Naheeda;Zhao, Tuanjie;Liu, Bin;Liu, Bingqiang;Liu, Bin;Zhao, Tuanjie

作者机构：

关键词： GmVPS8a; Soybean(Glycine max (L; ) Merr; ); Plant architecture; Transcriptome sequencing; Proteome sequencing; Auxin and sugar signaling

期刊名称：PLANT SCIENCE （ 影响因子：5.2； 五年影响因子：5.7 ）

ISSN： 0168-9452

年卷期： 2023 年 331 卷

页码：

收录情况： SCI

摘要： Vacuolar Protein Sorting 8 (Vps8) protein is a specific subunit of the class C core vacuole/endosome tethering (CORVET) complex that plays a key role in endosomal trafficking in yeast (Saccharomyces cerevisiae). However, its functions remain largely unclear in plant vegetative growth. Here, we identified a soybean (Glycine max) T4219 mutant characterized with compact plant architecture. Map-based cloning targeted to a candidate gene GmVPS8a (Glyma.07g049700) and further found that two nucleotides deletion in the first exon of GmVPS8a causes a premature termination of the encoded protein in the T4219 mutant. Its functions were validated by CRISPR/Cas9-engineered mutation in the GmVPS8a gene that recapitulated the T4219 mutant phenotypes. Furthermore, NbVPS8a-silenced tobacco (Nicotiana benthamiana) plants exhibited similar phenotypes to the T4219 mutant, suggesting its conserved roles in plant growth. The GmVPS8a is widely expressed in multiple organs and its protein interacts with GmAra6a and GmRab5a. Combined analysis of transcriptomic and prote-omic data revealed that dysfunction of GmVPS8a mainly affects pathways on auxin signal transduction, sugar transport and metabolism, and lipid metabolism. Collectively, our work reveals the function of GmVPS8a in plant architecture, which may extend a new way for genetic improvement of ideal plant-architecture breeding in soybean and other crops.

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