Ultra-sensitive self-powered patulin detection via CoOOH nanozyme-glucose oxidase bioconjugate and DNA walker-catalyzed nucleic acid frameworks

文献类型： 外文期刊

第一作者： Wang, Kaili

作者： Wang, Kaili;Jin, Chenchen;Wu, Qingnian;Ma, Yunzhi;Huang, Ke-Jing;Duan, Yun

作者机构：

关键词： Confined bioconjugate Catalysis; Enzyme-like activity; Patulin; Ultrasensitive detection

期刊名称：ANALYTICA CHIMICA ACTA （ 影响因子：6.0； 五年影响因子：5.7 ）

ISSN： 0003-2670

年卷期： 2025 年 1375 卷

页码：

收录情况： SCI

摘要： Background: Patulin, a hazardous mycotoxin widely present in moldy fruits, poses a serious threat to food safety and human health. The development of efficient detection methods has become a top priority. Current detection methods, however, are restricted by their poor portability and complicated operation procedures, which limit their extensive application and practical effectiveness in real-world food testing scenarios. Therefore, there is an urgent need for a more innovative and practical solution to address these challenges and improve the detection of patulin in food products. Results: This study addresses these challenges by developing an enzyme-free self-powered biosensor integrating two key innovations: a confined bioconjugate catalytic system combining CoOOH nanosheets (exhibiting peroxidase-like activity) with glucose oxidase (GOD), which enhances enzymatic stability and enables efficient glucose oxidation while eliminating hydrogen peroxide interference; a multi-legged DNA walker-driven catalytic hairpin assembly-hybridization chain reaction cascade amplification that generates three-dimensional DNA frameworks with enhanced methylene blue loading capacity. The biofuel cell-based platform achieves unprec-edented sensitivity through three synergistic mechanisms: Mg2+-activated DNA walker releases initiator strands through toehold-mediated strand displacement, triggering exponential nucleic acid amplification; CoOOH/GOD bioconjugate maintains 94.3 % initial activity after 48-h storage through it-it stacking and hydrogen bonding; DNA framework-modified biocathode demonstrates much higher redox current than linear DNA structures. Validation experiments reveal a linear detection range spanning 8 orders of magnitude (10-14-10-6 mg/mL) with a 9.05 x 10-15 mg/mL detection limit (S/N = 3). Banana sample analysis shows 94.0-109.0 % recovery rates with <5 % RSD. Significance: The developed biosensor offers a highly sensitive, portable, and operationally simple solution for patulin detection in real food samples. It effectively overcomes the limitations of traditional methods, providing a promising tool for ensuring food safety. The innovation and high performance of this biosensor hold great po-tential for practical applications in the food industry and related fields.

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