文献类型： 外文期刊
第一作者： Zhou, Xintao
作者： Zhou, Xintao;Fan, Jiaqi;Hu, Rongliang;Zhou, Xintao;Fan, Jiaqi;Zhang, Yanyan;Yang, Jinjin;Zhu, Rongnian;Yue, Huixian;Qi, Yu;Li, Qixuan;Wang, Yu;Chen, Teng;Zhang, Shoufeng;Hu, Rongliang;Zhou, Xintao;Fan, Jiaqi;Zhang, Yanyan;Yang, Jinjin;Zhu, Rongnian;Yue, Huixian;Qi, Yu;Li, Qixuan;Wang, Yu;Chen, Teng;Zhang, Shoufeng;Hu, Rongliang
作者机构：
关键词： African swine fever; African swine fever virus; E111R genes; virulence; dose-dependent
期刊名称：VIRUSES-BASEL （ 影响因子：4.7； 五年影响因子：4.8 ）
ISSN：
年卷期： 2023 年 15 卷 4 期
页码：
收录情况： SCI
摘要： African swine fever (ASF) is an acute infectious disease of domestic pigs and wild boars caused by the African swine fever virus (ASFV), with up to a 100% case fatality rate. The development of a vaccine for ASFV is hampered by the fact that the function of many genes in the ASFV genome still needs to be discovered. In this study, the previously unreported E111R gene was analyzed and identified as an early-expressed gene that is highly conserved across the different genotypes of ASFV. To further explore the function of the E111R gene, a recombinant strain, SY18 Delta E111R, was constructed by deleting the E111R gene of the lethal ASFV SY18 strain. In vitro, the replication kinetics of SY18 Delta E111R with deletion of the E111R gene were consistent with those of the parental strain. In vivo, high-dose SY18 Delta E111R (10(5.0) TCID50), administered intramuscularly to pigs, caused the same clinical signs and viremia as the parental strain (10(2.0) TCID50), with all pigs dying on days 8-11. After being infected with a low dose of SY18 Delta E111R (10(2.0) TCID50) intramuscularly, pigs showed a later onset of disease and 60% mortality, changing from acute to subacute infection. In summary, deletion of the E111R gene has a negligible effect on the lethality of ASFV and does not affect the viruses' ability to replicate, suggesting that E111R could not be the priority target of ASFV live-attenuated vaccine candidates.
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