Molecular cloning, characterization, and expression analysis of a Broad-Complex homolog during development in the oriental river prawn Macrobrachium nipponense

文献类型: 外文期刊

第一作者: Jiang, S. F.

作者: Jiang, S. F.;Sun, S. M.;Gong, Y. S.;Xiong, Y. W.;Qiao, H.;Zhang, W. Y.;Jin, S. B.;Fu, H. T.;Zhang, Y. P.

作者机构:

关键词: Macrobrachium nipponense;Broad-Complex;cDNA;Real-time quantitative polymerase chain reaction;Metamorphosis

期刊名称:GENETICS AND MOLECULAR RESEARCH ( 影响因子:0.764; 五年影响因子:0.912 )

ISSN: 1676-5680

年卷期: 2015 年 14 卷 2 期

页码:

收录情况: SCI

摘要: Broad-Complex (BR-C) is an early ecdysone-responsive gene encoding a family of zinc-finger transcription factors. In this study, we isolated the full-length cDNA of a BR-C homolog from the testes of the oriental river prawn (Macrobrachium nipponense), according to established expressed sequence tag information, using the rapid amplification of cDNA ends technique. The homolog was designated as MnBR-C. The full-length cDNA of MnBR-C contained a 1095-bp open reading frame encoding a precursor protein of 365 amino acid residues. Comparative and bioinformatic analyses revealed that MnBR-C exhibited a high degree of homology with BR-C proteins, and contained the BTB and Zf-H2C2-2 domains. Real-time quantitative polymerase chain reaction (qPCR) analysis revealed that the MnBR-C expression level varied significantly in the developing embryo, postembryonic larva, and adult tissue. Real-time qPCR showed that the MnBR-C gene was expressed in all of the tissues investigated, with the highest level of expression in the brain. In addition, MnBR-C was more abundantly expressed in the testes than in the ovaries.

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