Global changes in metabolic pathways in endocarp of 'Dayagan' hybrid citrus fruit during segment drying revealed by widely targeted metabolomics and transcriptomics analysis

文献类型： 外文期刊

第一作者： Hou, Jiao

作者： Hou, Jiao;Yan, Dandan;Liu, Yaci;Wang, Wei;Zeng, Kaifang;Yao, Shixiang;Hou, Jiao;Yan, Dandan;Liu, Yaci;Wang, Wei;Zeng, Kaifang;Yao, Shixiang;Hong, Min;He, Mingyang;Yang, Xiangzheng;Yang, Xiangzheng;Zeng, Kaifang;Yao, Shixiang

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关键词： Segment drying; Hybrid citrus fruit; Endocarp; Widely targeted metabolomics; Lignin synthesis pathway; Pectin metabolic pathway

期刊名称：POSTHARVEST BIOLOGY AND TECHNOLOGY （ 影响因子：7.0； 五年影响因子：6.9 ）

ISSN： 0925-5214

年卷期： 2023 年 198 卷

页码：

收录情况： SCI

摘要： Segment drying is a severe physiological disorder of citrus fruit that occurs during on-tree and postharvest storage. Although many research have focused on the analysis of metabolic pathways in juice vesicles during segment drying, the associated changes in endocarp are poorly understood. Physiological analysis, widely targeted metabolomics, and transcriptomics were utilized to systematically compare the changes in endocarp between normal and disordered fruit affected by segment drying in 'Dayagan' hybrid citrus. Physiological analysis showed that segment drying was associated with modification of phenotype, increase in cell wall components contents, and decrease in pectin degradation enzymes activities. Metabolomic profiling identified a total of 951 differentially accumulated metabolites upon segment drying. These metabolites were enriched in metabolic pathways relating to the synthesis of amino acids and derivatives, lipids, and flavonoids. Transcriptomics analysis revealed 6103 genes that were differentially expressed during segment drying, with increased expression of phenylpropanoid pathway genes and cell wall synthesis genes, while decreased expression of genes involved in pectin degradation. The global metabolic pathways that have been revealed suggest that the alteration of endocarp may be related to the water deficit stress in the pulp during segment drying. Finally, we carried out in vitro and in vivo assays, which proved that the endocarp could impede water loss of the pulp during segment drying. The present study is the first to offer insights into the metabolic changes that occur in the endocarp during segment drying, further indicating that water loss may be a key early event of the segment drying process.

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