Subcellular Distribution and Mechanism of Detoxifying Arsenic in Porphyra Yezoensis

文献类型： 外文期刊

第一作者： Shang De-Rong

作者： Shang De-Rong;Zhang Ji-Hong;Zhao Yan-Fang;Zhai Yu-Xiu;Ning Jin-Song;Ding Hai-Yan;Sheng Xiao-Feng;Duan Yuan-Hui

作者机构：

关键词： High performance liquid chromatography-inductively coupled plasma-mass spectrometry;Porphyra yezoensis;arsenite;Arsenate;Subcelluar distribution;Compartmentation

期刊名称：CHINESE JOURNAL OF ANALYTICAL CHEMISTRY （ 影响因子：1.134； 五年影响因子：0.909 ）

ISSN： 0253-3820

年卷期： 2013 年 41 卷 11 期

页码：

收录情况： SCI

摘要： In order to disclose the compartmentation of arsenic and the mechanism of detoxifying arsenic in Porphyra yezoensis, the transformation of different arsenic speciation and the subcellular distribution of arsenic in P. yezoensis were conducted. The seaweed was exposed to different concentrations (0.05, 0.1, 0.5 and 1.0 mg/L) of arsenite (As(III)) and arsenate (As(V)) for 6 days. Samples were randomly collected every day. The arsenic speciation in P. yezoensis was analyzed using HPLC-ICP-MS and the subcellular distribution of arsenic was conducted using differential centrifugation. Results showed that the arsenic accumulated in the seaweed increased with the exposed concentration and exposure time increasing. A clear dose-response was proved to exist in both As(III) and As(V) exposure, and the correlation coefficient was 0.9996 (p < 0.01) and 0.9844 (p < 0.01) for As(III) and As(V) respectively. In addition, the accumulation rate of As(III) was higher than that of As(V), and the accumulation coefficient of arsenic was 4719 and 4112 respectively for the seaweed exposed to 0.1mg/L As(III) and As(V) after 6ds exposure. The results also proved that there was a great difference in the different subcellular distribution and the compartmentation of arsenic in P. yezoensis. The sequcence of different subcellular distribution was cyplasmic supernatant (80%) > cell wall (15%) > cell organelles (5%). Cyplasmic supernatant was the main localization for arsenic and played an important role in the compartmentation of As. At the same time, the research of the arsenic transformation during accumulation showed that the absorbed As(III) in P. yezoensis would be quickly methylated and then be transformed to arsenic sugar (AsX1); the absorbed As(V) in P. yezoensis would be firstly reduced to As(III), which was then be methylated and transformed to arsenic sugar (AsX1). The ability of the compartmention and the transformation of different arsenic speciation in P. yezoensis may play a key role in detoxifying arsenic.

分类号：

  • 相关文献

[1]Phytochelatin synthesis in Dunaliella salina induced by arsenite and arsenate under various phosphate regimes. Wang, Ya,Zheng, Yanheng,Ge, Ying,Wang, Ya,Zhang, Chunhua.

[2]The oxidative transformation of sodium arsenite at the interface of alpha-MnO2 and water. Li, Xiu-juan,Liu, Cheng-shuai,Li, Fang-bai,Zhang, Li-jia,Liu, Chuan-ping,Li, Xiu-juan,Zhou, Yong-zhang,Li, Yong-tao,Zhang, Li-jia,Li, Xiu-juan. 2010

[3]Manipulating volatile emission in tobacco leaves by expressing Aspergillus niger beta-glucosidase in different subcellular compartments. Wei, S,Marton, I,Dekel, M,Shalitin, D,Lewinsohn, E,Bravdo, BA,Shoseyov, O.

[4]Transcriptional Regulation and Transport of Terpenoid Indole Alkaloid in Catharanthus roseus: Exploration of New Research Directions. Yang, Shihai,Liu, Jiaqi,Wang, Rui,Cai, Junjun. 2017

[5]Freezing tolerance of Porphyra yezoensis (Bangiales, Rhodophyta) gametophyte assessed by chlorophyll fluorescence. Zhu, Jian-Yi,Yao, Chun-Yan,Xu, Pu,Wang, Wen-Jun,Wang, Fei-Jiu,Sun, Xiu-Tao. 2011

[6]Intracellular compartmentation and membrane permeability to sugars and acids at different growth stages of peach. Jiang, Fengchao,Wang, Yuzhu,Ma, Lvyi,Jiang, Fengchao,Wang, Yuzhu,Sun, Haoyuan,Yang, Li,Zhang, Junhuan. 2013

[7]Bioremediation efficiency in the removal of dissolved inorganic nutrients by the red seaweed, Porphyra yezoensis, cultivated in the open sea. He, Peimin,Xu, Shannan,Zhang, Hanye,Wen, Shanshan,Dai, Yonjing,Zhang, Hanye,Lin, Senjie,Yarish, Charles. 2008

[8]Subcellular distribution and chemical forms of cadmium in the edible seaweed, Porphyra yezoensis. Zhao, Yanfang,Shang, Derong,Ning, Jinsong,Zhai, Yuxiu,Sheng, Xiaofeng,Ding, Haiyan,Wu, Jifa. 2015

[9]Cd accumulation and subcellular distribution in two ecotypes of Kyllinga brevifolia Rottb as affected by Cd treatments. Hao, Xiaoqing,Li, Tingxuan,Yu, Haiying,Zhang, Xizhou,Zheng, Zicheng,Chen, Guangdeng,Zhang, Shujin,Zhao, Li,Pu, Yong,Hao, Xiaoqing,Zhang, Shujin,Pu, Yong.

[10]Isolation and characterization of photosystem II from the filamentous sporophyte of Porphyra yezoensis. Wang, G. C.,Gao, Z. Q.,Meng, C. X.,Ye, N. H..

[11]Molecular Identification and Analysis of Arsenite Stress-Responsive miRNAs in Rice. Liu, Qingpo,Zhang, Hengmu.

[12]Arsenic speciation transformation and arsenite influx and efflux across the cell membrane of fungi investigated using HPLC-HG-AFS and, in-situ XANES. Zeng, Xibai,Su, Shiming,Wang, Xiurong,Li, Lianfang,Wang, Yanan,Wu, Cuixia,Bai, Lingyu,Duan, Ran,Feng, Qiufen,Zhang, Yangzhu,Zharig, Lili,Jiang, Sheng,Li, Aiguo. 2015

[13]A symbiotic bacterium differentially influences arsenate absorption and transformation in Dunaliella sauna under different phosphate regimes. Wang, Ya,Ge, Ying,Zhang, Chun Hua,Lin, Man Man.

[14]ARSENIC SPECIATION AND EFFECT OF ARSENATE INHIBITION IN A MICROCYSTIS AERUGINOSA CULTURE MEDIUM UNDER DIFFERENT PHOSPHATE REGIMES. Wang, Chang,Liu, Xi,Liu, Jiantong,Guo, Pengran,Wang, Chang,Gong, Yan. 2011

[15]Is the effect of silicon on rice uptake of arsenate (As-v) related to internal silicon concentrations, iron plaque and phosphate nutrition?. Guo, W.,Zhu, Y.-G.,Liu, W.-J.,Liang, Y.-C.,Geng, C.-N.,Wang, S.-G.. 2007

作者其他论文 更多>>

微信小程序