Pulsed Light Mutagenesis of the Willow Bracket Mushroom, Phellinus igniarius (Agaricomycetes), for Enhanced Production of Flavonoids, Laccase, and Fermentation Biomass

文献类型： 外文期刊

第一作者： Dong, Yating

作者： Dong, Yating;Ma, Haile;Qu, Wenjuan;Wang, Bei;Zhou, Cunshan;Dong, Yating;Gan, Bingcheng;Yan, Junjie;Han, Xing;Zhao, Jin;Feng, Rencai;Miao, Renyun;Golly, Moses Kwaku;Ye, Xiaofei;Zhang, Henan;Zhang, Henan;Zhang, Zhiyuan;Ma, Haile

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关键词： Phellinus igniarius; pulsed light; flavonoids; laccase activity; antagonistic; RAPD; medicinal mushrooms

期刊名称：INTERNATIONAL JOURNAL OF MEDICINAL MUSHROOMS （ 影响因子：1.706； 五年影响因子：1.817 ）

ISSN： 1521-9437

年卷期： 2022 年 24 卷 10 期

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收录情况： SCI

摘要： Phellinus igniarius is a medicinal fungus possessing potent therapeutic activity due to the polysaccharides, polyphenols, flavonoids, and other secondary metabolites they contain. Laccases are crucial enzymes involved in lignin degradation in Ph. igniarius and offer great potential to accomplish several bioprocesses. To generate Ph. igniarius strains with high biomass, flavonoid, and laccase activity, we used pulsed light (PL) technology for mutagenesis of Ph. igniarius protoplasts and screened for mutants with high biomass, flavonoid, and laccase activity. At the irradiation power of 100 J, treated distance 8.5 cm, irradiation frequency was 0.5 s/time, three times treatments, after five generations of selection, three mutants were obtained with higher biomass production. Compared with control, the mycelium biomass and the flavonoid production of the screened mutant strain QB72 were increased 20.87% and 53.51%, respectively. The total amount of the accumulated extracellular laccase of the QB72 in the first 6 and 8 days increased 23.38% and 22.37% respectively, and over the total 16 days it increased 9.62%. In addition, RAPD analysis results indicated that the genetic materials of the mutant QB72 were altered. PL mutagenesis method has great potential for developing strains, especially Phellinus.

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