Molecular cloning and expression analysis of two beta-defensin genes in the blunt snout bream (Megalobrama amblycephala)
文献类型: 外文期刊
第一作者: Liang, Tao
作者: Liang, Tao;Wang, Dan-Dan;Zhang, Gui-Rong;Wei, Kai-Jian;Wang, Wei-Min;Liang, Tao;Wang, Dan-Dan;Zhang, Gui-Rong;Wei, Kai-Jian;Zou, Gui-Wei
作者机构:
关键词: Antimicrobial peptide;beta-Defensins;Megalobrama amblycephala;Gene expression;Bacterial stimulation
期刊名称:COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY ( 影响因子:2.231; 五年影响因子:2.215 )
ISSN: 1096-4959
年卷期: 2013 年 166 卷 1 期
页码:
收录情况: SCI
摘要: beta-Defensins are a group of cysteine-rich, cationic antimicrobial peptides that play important roles in innate immune system against pathogenic microbes invading. In this study, the part-length cDNA sequences of two beta-defensin genes (maBD-1, maBD-2) in blunt snout bream (Megalobrama amblycephala) were identified. Homology analysis showed that the cDNA sequences of maBD-1 and maBD-2 had high similarities to those in common carp and zebrafish. Real-time quantitative PCR results exhibited that expression level of maBD-1 in juvenile tissues was the highest in skin, followed by blood and liver, whereas maBD-2 was lowly expressed in liver, kidney, brain and foregut In the early development period, fertilized eggs to 31-day post-hatching (dph) larvae, the expression levels of maBD-1 were higher at the stage from heart beat stage to 3 dph with the highest value at 1 dph, whereas maBD-2 was expressed higher at fertilized eggs and late cleavage stages. Following bacterial stimulation in vivo by Aeromonas sobria, maBD-2 expressions were significantly up-regulated in liver, skin, gill, and foregut of juveniles, and maBD-1 expressions were significantly up-regulated in liver and skin. The results suggest that maBD-1 and maBD-2 may play important roles in protecting blunt snout bream embryos, fry and juveniles from pathogenic microbe invading. (C) 2013 Elsevier Inc All rights reserved.
分类号:
- 相关文献
作者其他论文 更多>>
-
Reducing carbon and nitrogen loss by shortening the composting duration based on seed germination index (SCD@GI): Feasibilities and challenges
作者:Xiao, Ran;Li, Lan;Zhang, Yanye;Fang, Linfa;Su, Xiaoxuan;Xiao, Ran;Fang, Linfa;Su, Xiaoxuan;Li, Ronghua;Li, Ronghua;Song, Dan;Liang, Tao
关键词:Sustainable agriculture; Carbon and nitrogen loss control; Composting; Seed germination; Meta-analysis
-
A comprehensive continental-scale analysis of carbon footprint of food production: Comparing continents around the world
作者:Feng, Shi;Lakshmanan, Prakash;Zhang, Yi;Zhang, Tingting;Chen, Xinping;Wang, Xiaozhong;Lakshmanan, Prakash;Lakshmanan, Prakash;Lakshmanan, Prakash;Lakshmanan, Prakash;Liang, Tao;Zhang, Weijin
关键词:Food categories; Carbon footprint; Inter -continental comparisons; Total GHG emission
-
Positive regulation of the PhcB neighbouring regulator PrhX on expression of the type III secretion system and pathogenesis in Ralstonia solanacearum
作者:Huang, Jiajun;Guo, Tao;Zhang, Yong;Wang, Rongsheng;Zhang, Qi;Hikichi, Yasufumi;Ohnishi, Kouhei;Wang, Chunli;Liang, Tao;Jiang, Gaofei;Zhang, Yong
关键词:pathogenesis; PhcA; quorum sensing; Ralstonia solanacearum; type III secretion system
-
The Extension of Vegetable Production to High Altitudes Increases the Environmental Cost and Decreases Economic Benefits in Subtropical Regions
作者:Liang, Tao;Tao, Weilin;Wang, Yan;Zhou, Na;Hu, Wei;Zhang, Tao;Liao, Dunxiu;Chen, Xinping;Wang, Xiaozhong
关键词:life-cycle assessment; pepper; net ecosystem economic benefit; environmental cost; mitigation potential
-
Effects of in vitro simulated digestion and fecal fermentation of polysaccharides from straw mushroom (Volvariella volvacea) on its physicochemical properties and human gut microbiota
作者:Hu, Wei;Di, Qing;Liang, Tao;Zhou, Na;Chen, Hongxia;Zeng, Zhihong;Luo, Yang;Shaker, Majid;Shaker, Majid
关键词:Short -chain fatty acids; Volvariella volvacea polysaccharide; Gut microbiota; In vitro digestion; Fecal fermentation
-
Innovative nitrogen management strategy reduced N2O emission while maintaining high pepper yield in subtropical condition
作者:Zhang, Fen;Ma, Xiao;Cao, Hailing;Liu, Fabo;Wang, Junjie;Guo, Guangzheng;Liang, Tao;Chen, Xinping;Wang, Xiaozhong;Zhang, Fen;Ma, Xiao;Wang, Junjie;Chen, Xinping;Wang, Xiaozhong;Ma, Xiao;Ma, Xiao;Gao, Xiaopeng;Guo, Guangzheng;Liang, Tao;Wang, Yan;Chen, Xinping;Wang, Xiaozhong
关键词:Emission factor; Enhanced efficiency fertilizer; Nitrogen rate; Nitrous oxide; Vegetable; Yield
-
Optimized Nitrogen Fertilizer Rate Can Increase Yield and Nitrogen Use Efficiency for Open-Field Chinese Cabbage in Southwest China
作者:Cao, Hailin;Zhang, Fen;Wang, Junjie;Liu, Fabo;Guo, Guangzheng;Tian, Yiming;Liang, Tao;Chen, Xinping;Wang, Xiaozhong;Cao, Hailin;Zhang, Fen;Wang, Junjie;Liu, Fabo;Guo, Guangzheng;Tian, Yiming;Liang, Tao;Chen, Xinping;Wang, Xiaozhong;Fu, Jian;Ma, Xiao;Liang, Tao;Zhou, Na;Wang, Yan
关键词:Chinese cabbage; optimized N fertilizer rate; yield; N use efficiency; critical plant N concentration; critical root-zone soil N-min