Expression of lysine-rich protein gene and analysis of lysine content in transgenic wheat

文献类型： 外文期刊

第一作者： Meng, CM

作者： Meng, CM;Chen, XQ;Liang, RQ;Yang, FP;Zhang, LQ;Zhang, XD;Chen, TY;Sun, SSM

作者机构：

关键词： wheat (Triticum aestivum L.); lysine-rich protein; transgenic; expression; nutrition quality

期刊名称：CHINESE SCIENCE BULLETIN （ 影响因子：1.649； 五年影响因子：1.738 ）

ISSN： 1001-6538

年卷期： 2004 年 49 卷 19 期

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收录情况： SCI

摘要： Expression vector pBPC102, which carries winged bean lysine-rich protein (wblrp) gene and dihydropicolinate synthase (DHDPS) gene, was transferred into hexaploid winter wheat cv. Jinghua No.1, Jing411, You899 and Yangnong15 explants of immature inflorescence and immature embryos by particle bombardment. More than 100 transgenic plants were obtained under the selection of s-(2-aminoethyl)-L-cysteine (AEC). Confirmed transgenic plants of To and T-2 generation by PCR and PCR-Southern blotting analyses showed successful integration of wblrp gene into wheat genome. Analysis of transgenic plant lines of T-2 by Northern dot-blotting showed good expression of wblrp gene in offspring seed. The content of free lysine in leaves, contents of bound lysine and total proteins in seeds of T-2 transgenic wheat lines were determined and analyzed. Among 34 tested transgenic lines, levels of free lysine content in leaves of 9 transgenic lines are 2similar to3 times higher than un-transformed wild-type cultivars. Among 17 analyzed transgenic lines, bound lysine content of 4 transgenic lines is more than 10% higher than that of wild-type cultivars. Our research suggests that introducing wblrp gene into wheat is an effective way to improve its nutrition quality.

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