Phenotypic expression of bone-related genes in osteoblasts grown on calcium phosphate ceramics with different phase compositions
文献类型: 外文期刊
第一作者: Wang, CY
作者: Wang, CY;Duan, YR;Markovic, B;Barbara, J;Howlett, CR;Zhang, XD;Zreiqat, H
作者机构:
关键词: calcium phosphate ceramics;phase composition;cell proliferation;bone-related genes;phenotypic expression
期刊名称:BIOMATERIALS ( 影响因子:12.479; 五年影响因子:12.104 )
ISSN: 0142-9612
年卷期: 2004 年 25 卷 13 期
页码:
收录情况: SCI
摘要: Calcium phosphate ceramics with different hydroxyapatite (HA) and tricalcium phosphate (TCP) ratios have different chemical properties. Does the difference in phase composition affect osteoblast behavior? In this study, osteoblasts were cultured on 4 kinds of calcium phosphate ceramics, i.e. pure (HA), HT1 (HA/TCP, 70/30), HT2 (HA/TCP, 35/65), and pure TCP. Cell proliferation of SaOS-2 cells together with bone-related genes' mRNA expression and protein production in osteoblasts cultured on different calcium phosphate ceramics were detected at different time points. Data suggested that cell proliferation rate on TCP ceramics was lower than that on the other substrates tested. Generally, mRNA expressions for ostenectin and osteocalcin were similar among the four kinds of ceramics in most circumstances, whereas at six days, alkaline phosphatase mRNA expression was higher on HA and HT1 surfaces than on the other two materials. Collagen I mRNA expression was also affected by the phase composition of substrates. Osteocalcin and bone sialoprotein production in SaOS-2 cells was very similar no matter which ceramic surface the cells were grown upon. This study revealed that calcium phosphate ceramics substrate could support osteoblast growth and bone-related gene expression and its gene expression pattern explained the basis of the biocompatibility and bioactivity for calcium phosphate ceramics. (C) 2003 Published by Elsevier Ltd.
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