Vitamin D-3 modulates yellow catfish (Pelteobagrus fulvidraco) immune function in vivo and in vitro and this involves the vitamin D-3/VDR-type I interferon axis

文献类型： 外文期刊

第一作者： Cheng, Ke

作者： Cheng, Ke;Ma, Chunsong;Guo, Xun;Tang, Rong;Wang, Chunfang;Huang, Yanqing;Karrow, Niel A.

作者机构：

关键词： Yellow catfish; Vitamin D-3; Immune response; In vivo; In vitro; VD3/VDR-Type I interferon action axis

期刊名称：DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY （ 影响因子：3.636； 五年影响因子：3.654 ）

ISSN： 0145-305X

年卷期： 2020 年 107 卷

页码：

收录情况： SCI

摘要： Vitamin D-3 (VD3) has been shown to regulate immune function in mammals. 1,25-dihydroxyvitamin (1,25(OH)(2)D-3) is the active form of vitamin D-3, which is also known as calcitriol. The current study investigated the immunomodulatory effects of 1,25(OH)(2)D-3 on the innate immune response of yellow catfish (Pelteobagrus fulvidraco) after in vivo and in vitro immune challenge. The in vivo results showed that increasing dietary vitamin D-3 decreased mortality, enhanced the immune protective rate, and increased serum lysozyme, catalase and SOD activities in yellow catfish infected with Edwardsiella Ictaluri (p < 0.05). The in vitro results showed that 1,25(OH)(2)D-3 (0, 1, 10, 100, 200 pM) dose-dependently attenuated the rate of apoptosis and production or reactive oxygen species and increased the phagocytic activity of head kidney macrophages stimulated with 10 mg/L lipopolysaccharide (LPS) and 100 mg/L of Poly(I:C) (p < 0.05). Real-time quantitative PCR results showed that increasing dietary vitamin D3 content in vivo and increasing the level of 1,25(OH)(2)D-3 in vitro partially regulated the expression of VD3/VDR-type I interferon axis genes (vdr, irf-3, ifn-a, jak1, stat1, ifi56 and ifp35) after immune challenge. These results indicated that vitamin D-3 content helped yellow catfish to resist oxidative stress and inflammation caused by immune challenge, and immunomodulation involved the VD3/VDRtype I interferon action axis.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序