Development of haemagglutination assay for titration of porcine circovirus type 2

文献类型： 外文期刊

第一作者： Cheng, Haiwei

作者： Cheng, Haiwei;Qiao, Xuwen;Du, Luping;Hou, Jibo;Chen, Jin;Zheng, Qisheng;Cheng, Haiwei;Qiao, Xuwen;Du, Luping;Hou, Jibo;Chen, Jin;Zheng, Qisheng;Cheng, Haiwei;Qiao, Xuwen;Du, Luping;Hou, Jibo;Chen, Jin;Zheng, Qisheng;Cheng, Haiwei;Qiao, Xuwen;Du, Luping;Hou, Jibo;Chen, Jin;Zheng, Qisheng;Yang, Li;Cai, Zizheng

作者机构：

关键词： PCV2; Titration; Hemagglutination; Chicken red blood cells; Bispecific nanobody

期刊名称：ANALYTICAL BIOCHEMISTRY （ 影响因子：3.365； 五年影响因子：3.049 ）

ISSN： 0003-2697

年卷期： 2020 年 598 卷

页码：

收录情况： SCI

摘要： Porcine circovirus type 2 (PCV2) was one of the most economically important viral pathogens in all the swineproducing countries and often resulted in tremendous economic losses for the swine industry. As PCV2 could not cause cytopathogenic effects while propagated in infected cells, many complicated experiments should be performed to titrate its virus titer. In this study we developed a simple and effective hemagglutination assay for titration of virus titer of PCV2. To develop the hemagglutination assay, a recombinant bispecific nanobody (BsNb) against PCV2 and chicken red blood cells (cRBCs) was constructed based on two nanobodies (NbPCV11 and NbRBC48) which were selected from the non-immunized nanobody library, respectively. The hemagglutination assay was used to titrate the virus titer of PCV2 propagated in cell culture by simple naked-eye observation within 30 min, with the detection limit of 10(4.09) tissue culture infective dose 50 (TCID50)/mL, excellent specificity and reproducibility. Therefore, the hemagglutination assay had potential to be a rapid, reliable, cost-effective, user-friendly qualitative and semi-quantitative tool for titration of virus titer of PCV2 during the vaccine manufacturing process.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序