Candidate resistance genes selection and transcriptome analysis for the early responses to Plasmopara viticola infection in grape cultivars

文献类型： 外文期刊

第一作者： Liu, Li

作者： Liu, Li;Zhang, Bo;Huang, Yu Fei;Liu, Li;Wang, Hui;Yu, Shu Yi;Guan, Tian Shu;Liu, Chang Yuan;Zhang, Bo

作者机构：

关键词： Downy mildew; RNA sequencing; Differentially expressed gene; Defense response

期刊名称：JOURNAL OF PLANT PATHOLOGY （ 影响因子：1.729； 五年影响因子：1.681 ）

ISSN： 1125-4653

年卷期： 2020 年 102 卷 3 期

页码：

收录情况： SCI

摘要： Grape downy mildew (Plasmopara viticola) is one of the most destructive diseases of grapevine worldwide. In order to better understand the cellular processes involved in resistance to downy mildew, leaves of Vitis vinifera cv. 'Centennial Seedless' (susceptible) and Vitis labruscaxVitis riparia cv. 'Beta' (resistant) were inoculated with Plasmopara viticola, and were used in the Illumina HiSeq (TM) 2000 platform for deep transcriptome sequencing. We performed transcriptome analysis and identified a total of 1091 differentially expressed genes (DEGs) in the disease-resistant cultivar and 849 in the susceptible cultivar. To increase understanding of the DEGs, the datasets were analyzed using Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genome (KEGG) database. Most of them were found to be associated with plant-pathogen interaction, flavonoid synthesis, phenylpropanoid synthesis metabolism and phytohormone signal transduction. Moreover, transcription factors ERF, MYB, WRKY, and bHLH associated with disease resistance were screened. A total of 196 genes were identified as the candidate resistant genes. The expression of 18 differentially expressed genes was detected by RT-qPCR. Finally, the pattern of differentially expressed genes was consistent with the result of transcriptome sequencing. The present study identified several candidate resistance genes and signal transduction pathways that may contribute to downy mildew resistance in grapes.

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