Identification of Gentiana rigescens from different geographical origins based on HPLC and FTIR fingerprints

文献类型： 外文期刊

第一作者： Zhao, Yanli

作者： Zhao, Yanli;Yuan, Tianjun;Wu, Lihua;Zhang, Ji;Zuo, Zhitian;Wang, Yuanzhong

作者机构：

期刊名称：ANALYTICAL METHODS （ 影响因子：2.896； 五年影响因子：2.716 ）

ISSN： 1759-9660

年卷期： 2020 年 12 卷 17 期

页码：

收录情况： SCI

摘要： Gentiana rigescens is a traditional Chinese medicine with efficacy in liver protection, as a cholagogic, anti-hyperglycemic, and anti-hypertension agent, and in relieving spasms and pain. The geographical environments of different locations are very complicated, with different soils, climates, sun exposure, etc.; this has a remarkable effect on the quality of medicinal herbs. To identify different geographic origins of G. rigescens, we analyzed the high-performance liquid chromatography (HPLC) and Fourier transform infrared spectroscopy (FTIR) fingerprints of G. rigescens with the aid of chemometrics. Five variable selection methods, including competitive adaptive reweighted sampling (CARS), random frog (RF), subwindow permutation analysis (SPA), Monte Carlo uninformative variable elimination (MC-UVE) and genetic algorithms (GA), were used to screen the characteristic variables for both the HPLC and FTIR fingerprints. Then, the corresponding partial least squares discriminant analysis (PLS-DA) models were built. The results showed that for both HPLC and FTIR, the GA-PLS-DA models were the most robust models for identification of the geographical origins of G. rigescens, which indicated that the recognition results of HPLC and FTIR are consistent. Through HPLC-FTIR two-dimensional (2D) hetero-correlation analysis, the results showed a substantial correlation between HPLC and FTIR, which suggests that spectral correlative chromatography for multicomponent comparison can be conducted. In terms of G. rigescens, qualitative identification using FTIR is more convenient, rapid, environment-friendly and inexpensive than that using HPLC.

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