Fur Seal Feces-Associated Circular DNA Virus Identified in Pigs in Anhui, China

文献类型： 外文期刊

第一作者： Shi, Zhibin

作者： Shi, Zhibin;Liu, Chunguo;Yang, Huanliang;Chen, Yan;Wei, Lili;Liu, Zaisi;Jiang, Yongping;He, Xijun;Wang, Jingfei;Shi, Zhibin;Liu, Chunguo;Yang, Huanliang;Chen, Yan;Wei, Lili;Liu, Zaisi;Jiang, Yongping;He, Xijun;Wang, Jingfei;Liu, Hua;Liu, Hua

作者机构：

关键词： Fur seal feces-associated circular DNA virus (FSfaCV); Viral metagenomics; Pig; Genomic characterization; China

期刊名称：VIROLOGICA SINICA （ 影响因子：4.327； 五年影响因子：4.08 ）

ISSN： 1674-0769

年卷期：

页码：

收录情况： SCI

摘要： Fur seal feces-associated circular DNA virus (FSfaCV) is an unclassified circular replication-associated protein (Rep)-encoding single-stranded (CRESS) DNA virus that has been detected in mammals (fur seals and pigs). The biology and epidemiology of the virus remain largely unknown. To investigate the virus diversity among pigs in Anhui Province, China, we pooled 600 nasal samples in 2017 and detected viruses using viral metagenomic methods. From the assembled contigs, 12 showed notably high nucleotide acid sequence similarities to the genome sequences of FSfaCVs. Based on these sequences, a full-length genome sequence of the virus was then obtained using overlapping PCR and sequencing, and the virus was designated as FSfaCV-CHN (GenBank No. MK462122). This virus shared 91.3% and 90.9% genome-wide nucleotide sequence similarities with the New Zealand fur seal strain FSfaCV-as50 and the Japanese pig strain FSfaCV-JPN1, respectively. It also clustered with the two previously identified FSfaCVs in a unique branch in the phylogenetic tree based on the open reading frame 2 (ORF2), Rep-coding gene, and the genome of the reference CRESS DNA viruses. Further epidemiological investigation using samples collected in 2018 showed that the overall positive rate for the virus was 56.4% (111/197) in Anhui Province. This is the first report of FSfaCVs identified in pigs in China, and further epidemiological studies are warranted to evaluate the influence of the virus on pigs.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序