Frameshift mutation in myostatin gene by zinc-finger nucleases results in a significant increase in muscle mass in Meishan sows

文献类型： 外文期刊

第一作者： Bi, Hanfang

作者： Bi, Hanfang;Xie, Shanshan;Cai, Chunbo;Jiang, Shengwang;Xiao, Gaojun;Li, Biao;Li, Xiang;Cui, Wentao;Qian, Lili;Xie, Shanshan

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关键词： MSTN; ZFN-edited; skeletal muscle; lean meat yield; myofibres

期刊名称：CZECH JOURNAL OF ANIMAL SCIENCE （ 影响因子：1.267； 五年影响因子：1.428 ）

ISSN： 1212-1819

年卷期： 2020 年 65 卷 5 期

页码：

收录情况： SCI

摘要： Myostatin (MSTN) is a negative regulator of skeletal muscle growth and development. A significant increase in skeletal muscle was observed in MstN( -/-) mice compared with wild-type mice. So far, there has been no report on porcine MSTN mutations leading to skeletal muscle hypertrophy. In this report a MSTN frameshift mutation missing 11 nucleotides in exon 2 was introduced into Meishan pigs by zinc finger nuclease (ZFN) technology. ZFN-edited MSTN -/- Meishan pigs were successfully produced by a cloning method of somatic cell nucleus transfer. Results from slaughter experiments indicated that lean meat yield increased 16.53% in about 80 kg (10-months-old) MSTN-/- Meishan sows compared with their corresponding wild-type counterparts. The lean percentage of carcass from MSTN-/- sows was 61.20% vs 48.25% for MSTN-/- sows and 44.67% for wild-type sows. The fat of MSTN -/- sows was significantly lower than that of MSTN+/- and wild-type sows. The loin eye area of MSTN -/- Meishan sows (56.42 cm(2)) was greater than that of MSTN+/- (37.39 cm(2)) and wild-type (26.26 cm(2)) sows. The muscle fibre area of longissimus muscle in wild-type Meishan sows (1 946 mu m(2)) was significantly greater than that of MSTN+/- (1 324 mu m( 2) ) and MSTN-/- (1 419 mu m(2)) sows. Moreover the significantly increased skeletal muscle in these MSTN-/- Meishan sows was mainly due to the increase in the number of myofibres rather than to hypertrophy. Compared with wild-type Meishan sows, it was noted that myofibres had transformed from type I to IIB in MSTN-/- Meishan sows. Our present study demonstrated that frameshift mutation in MSTN by ZFN technology led to a significant increase in muscle mass and a significant decrease in fat content in Meishan sows.

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