Immune effect of a Newcastle disease virus DNA vaccine with IL-12 as a molecular adjuvant delivered by electroporation

文献类型： 外文期刊

第一作者： Xie, Peng

作者： Xie, Peng;Li, Yanling;Liang, Jianpeng;Xiang, Bin;Lin, Qiuyan;Jin, Jiaqi;Xu, Chenggang;Ren, Tao;Xie, Peng;Li, Yanling;Liang, Jianpeng;Xiang, Bin;Lin, Qiuyan;Jin, Jiaqi;Xu, Chenggang;Ren, Tao;Xie, Peng;Li, Yanling;Liang, Jianpeng;Xiang, Bin;Lin, Qiuyan;Jin, Jiaqi;Xu, Chenggang;Ren, Tao;Li, Yaling;Ding, Chan

作者机构：

期刊名称：ARCHIVES OF VIROLOGY （ 影响因子：2.574； 五年影响因子：2.466 ）

ISSN： 0304-8608

年卷期： 2020 年 165 卷 9 期

页码：

收录情况： SCI

摘要： Newcastle disease (ND), caused by virulent Newcastle disease virus (NDV) strains, has been one of the most problematic diseases affecting the poultry industry worldwide. Conventional vaccines provide effective protection for birds to survive ND outbreaks, but they may not completely suppress NDV shedding. NDV strains circulate on farms for a long time after the initial infection and cause potential risks. A new vaccine with fast clearance ability and low viral shedding is needed. In this study, we used interleukin-12 (IL-12) as an adjuvant and electroporation (EP) as an advanced delivery system to improve a DNA vaccine candidate. The fusion (F) protein gene from an NDV strain of the prevalent genotype VII.1.1 was cloned to prepare the vaccine. Chickens immunized with the F gene DNA vaccine co-delivered with an IL-12-expressing plasmid DNA showed higher neutralizing antibody levels and stronger concanavalin-A-induced lymphocyte proliferation than those treated with the F gene DNA vaccine alone. The co-delivered vaccine provided 100% protection, and less viral shedding and a shorter release time were observed in challenged chickens than when the F gene DNA vaccine was administered alone. The use of F gene DNA combined with IL-12 delivered by electroporation is a promising approach for vaccination against ND.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序