A Novel miRNA-hlo-miR-2-Serves as a Regulatory Factor That Controls Molting Events by Targeting CPR1 inHaemaphysalis longicornisNymphs

文献类型： 外文期刊

第一作者： Liu, Wen-Ge

作者： Liu, Wen-Ge;Luo, Jin;Ren, Qiao-Yun;Qu, Zhi-Qiang;Xu, Xiao-Feng;Ni, Jun;Rashid, Muhammad;Wu, Ze-Gong;Tan, Yang-Chun;Qiu, Xiao-Fei;Luo, Jian-Xun;Yin, Hong;Wang, Hui;Liu, Guang-Yuan;Liu, Wen-Ge;Yang, Zeng-Qi;Xiao, Sa;Lin, Han-Liang;Xiao, Rong-Hai;Chen, Rong-Gui;Yin, Hong;Wang, Hui

作者机构：

关键词： H; longicornis; hlo-miR-2; cuticular protein; molting event; RNA interference

期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：5.64； 五年影响因子：6.32 ）

ISSN： 1664-302X

年卷期： 2020 年 11 卷

页码：

收录情况： SCI

摘要： Successful completion of the molting process requires new epidermal growth and ecdysis of the old cuticle inHaemaphysalis longicornis(H. longicornis). MicroRNAs (miRNAs) participate in the development of organisms by inhibiting the expression of their target mRNAs. In this study, a novel tick-specific miRNA was identified and denoted hlo-miR-2 that serves as a novel regulator of molting events inH. longicornisnymphs by targeting a cuticular protein. The full length of this cuticular protein was first obtained and named it CPR1. A qRT-PCR analysis showed that hlo-miR-2 and CPR1 exhibit significant tissue and temporal specificity and that their transcription levels are negatively correlated during the molting process. CPR1, as a direct target of hlo-miR-2, was identified by a luciferase reporter assayin vitro. Agomir treatment indicated that the overexpression of hlo-miR-2 significantly reduced the protein expression level of CPR1, decreased the molting rate and delayed the molting time point inH. longicornisnymphs. RNA interference (RNAi) experiments demonstrated that CPR1 was significantly associated with the molting process inH. longicornisnymphs. Phenotypic rescue experiments convincingly showed that hlo-miR-2 participated in molting events by targeting CPR1 inH. longicornisnymphs. In summary, we present evidence demonstrating that miRNAs constitute a novel important regulator of molting events in addition to hormones. The described functional evidence implicating CPR1 in molting events contributes to an improved understanding of the distinct functions of the CPR family in ticks and will aid the development of a promising application of cuticular protein RNAi in tick control.

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