The Genome Analysis ofMethylobacterium populiYC-XJ1 with Diverse Xenobiotics Biodegrading Capacity and Degradation Characteristics of Related Hydrolase

文献类型： 外文期刊

第一作者： Li, Xianjun

作者： Li, Xianjun;Wang, Junhuan;Jia, Yang;Yan, Yanchun;Reheman, Aikebaier

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关键词： Methylobacterium populi; genome sequencing; hydrolase; quizalofop-p-ethyl; diethyl phthalate; degradation

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）

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年卷期： 2020 年 21 卷 12 期

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收录情况： SCI

摘要： Methylobacterium populiYC-XJ1 isolated from desert soil exhibited a diverse degrading ability towards aromatic oxyphenoxypropionic acid esters (AOPPs) herbicide, phthalate esters (PAEs), organophosphorus flame retardants (OPFRs), chlorpyrifos and phoxim. The genome of YC-XJ1 was sequenced and analyzed systematically. YC-XJ1 contained a large number of exogenous compounds degradation pathways and hydrolase resources. The quizalofop-p-ethyl (QPE) degrading geneqpeh2and diethyl phthalate (DEP) degrading genedeph1were cloned and expressed. The characteristics of corresponding hydrolases were investigated. The specific activity of recombinant QPEH2 was 0.1 +/- 0.02 U mg(-1)for QPE withk(cat)/K(m)values of 1.8 +/- 0.016 (mM(-1)center dot s(-1)). The specific activity of recombinant DEPH1 was 0.1 +/- 0.02 U mg(-1)for DEP withk(cat)/K(m)values of 0.8 +/- 0.02 (mM(-)(1)center dot s(-)(1)). This work systematically illuminated the metabolic versatility of strain YC-XJ1 via the combination of genomics analysis and laboratory experiments. These results suggested that strain YC-XJ1 with diverse xenobiotics biodegrading capacity was a promising candidate for the bioremediation of polluted sites.

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