Functional divergence of two duplicatedFertilization Independent Endospermgenes in rice with respect to seed development

文献类型： 外文期刊

第一作者： Cheng, Xiaojun

作者： Cheng, Xiaojun;Pan, Meiyao;Zhou, Yong;Niu, Baixiao;Chen, Chen;E, Zhiguo

作者机构：

关键词： cellularization; dormancy; endosperm development; function divergence; polycomb repressive complex 2; rice

期刊名称：PLANT JOURNAL （ 影响因子：6.417； 五年影响因子：7.627 ）

ISSN： 0960-7412

年卷期： 2020 年 104 卷 1 期

页码：

收录情况： SCI

摘要： Fertilization Independent Endosperm (FIE) is an essential member of Polycomb Repressive Complex 2 (PRC2) that plays important roles in the developmental regulation of plants.OsFIE1andOsFIE2are twoFIEhomologs in the rice genome. Here, we showed thatOsFIE1probably duplicated fromOsFIE2after the origin of the tribe Oryzeae, but has a specific expression pattern and methylation landscape. During evolution,OsFIE1underwent a less intensive purifying selection than didOsFIE2. The mutantosfie1produced smaller seeds and displayed reduced dormancy, indicating thatOsFIE1predominantly functions in late seed development. Ectopic expression ofOsFIE1, but notOsFIE2, was deleterious to vegetative growth in a dose-dependent manner. The newly evolved N-terminal tail of OsFIE1 was probably not the cause of the adverse effects on vegetative growth. The CRISPR/Cas9-derived mutantosfie2exhibited impaired cellularization of the endosperm, which suggested thatOsFIE2is indispensable for early seed development as a positive regulator of cellularization. Autonomous endosperm was observed in bothOsFIE2(+-)andosfie1/OsFIE2(+-)but at a very low frequency. Although OsFIE1-PRC2 exhibited H3K27me3 methyltransferase ability in plants, OsFIE1-PRC2 is likely to be less important for development in rice than is OsFIE2-PRC2. Our findings revealed the functional divergence ofOsFIE1andOsFIE2and shed light on their distinct evolution following duplication.

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