Sensitive Immunochromatographic Assay Using Highly Luminescent Quantum Dot Nanobeads as Tracer for the Detection of Cyproheptadine Hydrochloride in Animal-Derived Food

文献类型： 外文期刊

第一作者： Li, Pan

作者： Li, Pan;Liu, Beibei;Wu, Qin;Wang, Yulong;Dong, Sa;Zhang, Hanxiaoya;Zhang, Cunzheng;Yang, Cuifeng;Wu, Qin;Zhang, Cunzheng;Dong, Sa;Vasylieva, Natalia;Hammock, Bruce D.;Vasylieva, Natalia;Hammock, Bruce D.

作者机构：

关键词： immunochromatographic assay; quantum dot nanobeads; cyproheptadine hydrochloride; animal-derived foods; monoclonal antibody

期刊名称：FRONTIERS IN CHEMISTRY （ 影响因子：5.221； 五年影响因子：5.385 ）

ISSN： 2296-2646

年卷期： 2020 年 8 卷

页码：

收录情况： SCI

摘要： Cyproheptadine hydrochloride (CYP), used as human and veterinary drug, has been used illegally as feed additive for food-producing animals, which could remain in food and jeopardize human health. There is a need for on-site detection of CYP residue in animal-derived food. In this study, a hapten was designed, and a specific monoclonal antibody (mAb) was developed to detect CYP with an IC(50)of 1.38 ng/mL and negligible cross-reactivity (CR) for other analogs. Forthermore, a high sensitive immunochromatographic assay (QBs-ICA) was developed using quantum dot nanobeads as reporters. The assay showed the linear detection range (IC20-IC80) of 0.03-0.52 ng/mL, the limit of detection (LOD) and visual detection limit (VDL) reached to 0.01 and 0.625 ng/mL, respectively. Spiked recovery study in pig urine and pork confirmed that the QBs-ICA was applicable for on-site testing. This assay showed better sensitivity and speedy than the reported instrumental analysis and immunoassays.

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