Genome-Wide Characterization and Expression Analysis of NHX Gene Family under Salinity Stress inGossypium barbadenseand Its Comparison withGossypium hirsutum

文献类型： 外文期刊

第一作者： Akram, Umar

作者： Akram, Umar;Song, Yuhan;Liang, Chengzhen;Abid, Muhammad Ali;Askari, Muhammad;Myat, Aye Aye;Abbas, Mubashir;Guo, Sandui;Zhang, Rui;Meng, Zhigang;Malik, Waqas;Ali, Zulfiqar

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关键词： G; barbadense; GbNHX; phylogenetic; abiotic stress; Na+; H(+)antiporter; amiloride; Cis-elements

期刊名称：GENES （ 影响因子：4.096； 五年影响因子：4.339 ）

ISSN：

年卷期： 2020 年 11 卷 7 期

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收录情况： SCI

摘要： Cotton is an important economic crop affected by different abiotic stresses at different developmental stages. Salinity limits the growth and productivity of crops worldwide. Na+/H(+)antiporters play a key role during the plant development and in its tolerance to salt stress. The aim of the present study was a genome-wide characterization and expression pattern analysis under the salinity stress of the sodium-proton antiporter (NHX) ofGossypium barbadensein comparison withGossypium hirsutum. InG. barbadense, 25NHXgenes were identified on the basis of the Na+_H(+)exchanger domain. All except one of theG. barbadenseNHXtransporters have an Amiloride motif that is a known inhibitor of Na(+)ions in plants. A phylogenetic analysis inferred three classes ofGbNHXgenes-viz., Vac (GbNHX1,2and4), Endo (GbNHX6), and PM (GbNHX7). A high number of the stress-relatedcis-acting elements observed in promoters show their role in tolerance against abiotic stresses. The Ka/Ks values show that the majority ofGbNHXgenes are subjected to strong purifying selection under the course of evolution. To study the functional divergence ofG. barbadenseNHXtransporters, the real-time gene expression was analyzed under salt stress in the root, stem, and leaf tissues. InG. barbadense, the expression was higher in the stem, while inG. hirsutumthe leaf and root showed a high expression. Moreover, our results revealed thatNHX2homologues in both species have a high expression under salinity stress at higher time intervals, followed byNHX7. The protein-protein prediction study revealed thatGbNHX7is involved in the CBL-CIPK protein interaction pathway. Our study also provided valuable information explaining the molecular mechanism of Na(+)transport for the further functional study of GossypiumNHXgenes.

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