Proteome-Wide Analyses Provide New Insights into the Compatible Interaction of Rice with the Root-Knot NematodeMeloidogyne graminicola

文献类型： 外文期刊

第一作者： Xiang, Chao

作者： Xiang, Chao;Peng, Deliang;Kang, Houxiang;Liu, Maoyan;Huang, Wenkun;Liu, Shiming;Yang, Xiaoping;Liu, Maoyan;Li, Wei

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关键词： rice; root-knot nematode; Meloidogyne graminicola; compatible interaction; proteome-wide analyses

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）

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年卷期： 2020 年 21 卷 16 期

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收录情况： SCI

摘要： The root-knot nematodeMeloidogyne graminicolais an important pathogen in rice, causing huge yield losses annually worldwide. Details of the interaction between rice andM. graminicolaand the resistance genes in rice still remain unclear. In this study, proteome-wide analyses of the compatible interaction of thejaponicarice cultivar "Nipponbare" (NPB) withM. graminicolawere performed. In total, 6072 proteins were identified in NPB roots with and without infection ofM. graminicolaby label-free quantitative mass spectrometry. Of these, 513 specifically or significantly differentially expressed proteins were identified to be uniquely caused by nematode infection. Among these unique proteins, 99 proteins were enriched on seven Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. By comparison of protein expression and gene transcription, LOC_Os01g06600 (ACX, a glutaryl-CoA dehydrogenase), LOC_Os09g23560 (CAD, a cinnamyl-alcohol dehydrogenase), LOC_Os03g39850 (GST, a glutathione S-transferase) and LOC_Os11g11960 (RPM1, a disease resistance protein) on the alpha-linolenic acid metabolism, phenylpropanoid biosynthesis, glutathione metabolism and plant-pathogen interaction pathways, respectively, were all associated with disease defense and identified to be significantly down-regulated in the compatible interaction of NPB with nematodes, while the corresponding genes were remarkably up-regulated in the roots of a resistant rice accession "Khao Pahk Maw" with infection of nematodes. These four genes likely played important roles in the compatible interaction of rice withM. graminicola. Conversely, these disease defense-related genes were hypothesized to be likely involved in the resistance of resistant rice lines to this nematode. The proteome-wide analyses provided many new insights into the interaction of rice withM. graminicola.

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