RiceEARLY SENESCENCE 2, encoding an inositol polyphosphate kinase, is involved in leaf senescence

文献类型： 外文期刊

第一作者： Yang, Shenglong

作者： Yang, Shenglong;Zhang, Anpeng;Zhang, Yu;Xu, Zhengjin;Wang, Jiayu;Yang, Shenglong;Fang, Guonan;Zhang, Anpeng;Ruan, Banpu;Jiang, Hongzhen;Ding, Shilin;Liu, Chaolei;Zhang, Yu;Jaha, Noushin;Hu, Peng;Gao, Zhenyu;Qian, Qian

作者机构：

关键词： Early senescence; es2; OsIPK2; Inositol polyphosphate kinase; Rice (Oryza sativa L; )

期刊名称：BMC PLANT BIOLOGY （ 影响因子：4.215； 五年影响因子：4.96 ）

ISSN： 1471-2229

年卷期： 2020 年 20 卷 1 期

页码：

收录情况： SCI

摘要： Background Early leaf senescence influences yield and yield quality by affecting plant growth and development. A series of leaf senescence-associated molecular mechanisms have been reported in rice. However, the complex genetic regulatory networks that control leaf senescence need to be elucidated. Results In this study, anearly senescence 2(es2) mutant was obtained from ethyl methanesulfonate mutagenesis (EMS)-induced mutational library for theJaponicarice cultivar Wuyugeng 7 (WYG7). Leaves ofes2showed early senescence at the seedling stage and became severe at the tillering stage. The contents of reactive oxygen species (ROS) significantly increased, while chlorophyll content, photosynthetic rate, catalase (CAT) activity significantly decreased in thees2mutant. Moreover, genes which related to senescence, ROS and chlorophyll degradation were up-regulated, while those associated with photosynthesis and chlorophyll synthesis were down-regulated ines2mutant compared to WYG7. TheES2gene, which encodes an inositol polyphosphate kinase (OsIPK2), was fine mapped to a 116.73-kb region on chromosome 2. DNA sequencing ofES2in the mutant revealed a missense mutation, ES2 was localized to nucleus and plasma membrane of cells, and expressed in various tissues of rice. Complementation test and overexpression experiment confirmed thatES2completely restored the normal phenotype, with chlorophyll contents and photosynthetic rate increased comparable with the wild type. These results reveal the new role ofOsIPK2in regulating leaf senescence in rice and therefore will provide additional genetic evidence on the molecular mechanisms controlling early leaf senescence. Conclusions TheES2gene, encoding an inositol polyphosphate kinase localized in the nucleus and plasma membrane of cells, is essential for leaf senescence in rice. Further study ofES2will facilitate the dissection of the genetic mechanisms underlying early leaf senescence and plant growth.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序