EngineeringNicotiana tabacumfor thede novobiosynthesis ofDMNTto regulate orientation behavior of the parasitoid waspsMicroplitis mediator

文献类型： 外文期刊

第一作者： Liu, Danfeng

作者： Liu, Danfeng;An, Xingkui;Ghasemzadeh, Somayyeh;Huang, Xinzheng;Kou, Junfeng;Sun, Peiyao;Zhang, Yongjun;Liu, Danfeng;Li, Wei;Li, Wei;Chen, Jieyin

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关键词： DMNT biosynthesis; co-expression ofGhCYP82Ls andGhTPS14; transgenic tobacco; Microplitis mediator; orientation behavior

期刊名称：PEST MANAGEMENT SCIENCE （ 影响因子：4.845； 五年影响因子：4.674 ）

ISSN： 1526-498X

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收录情况： SCI

摘要： BACKGROUND (E)-4,8-dimethylnona-1,3,7-triene (DMNT), one of the homoterpenes, is thought to contribute to plant indirect defense against insect herbivores. DMNT-enriched plants have great application potential to regulate insect behavior in the 'push & pull' strategy of pest management. However,de novobiosynthesis of DMNT in plants without a homoterpene metabolic pathway in their wild type is still not achieved, and the role of DMNT played in these plants and their interacted insects remains unclear. RESULTS Cytochrome P450s and terpene synthases involved in homoterpenes biosynthesis in cotton plants were employed to generate DMNT-releasing tobacco plants. SingleGhTPS14 transgenicNicotiana tabacumonly emitted (E)-nerolidol, the precursor of DMNT. Transgenic tobaccos expressing singleGhCYP82Ls were unable to produce DMNT or TMTT, while DMNT was detected when exogenous (E)-nerolidol was added. Compared to wild-type plants, only co-expression ofGhCYP82Ls andGhTPS14 in transgenic tobaccos triggered the constitutive release of single-component DMNT. Furthermore, DMNT-emitting transgenic tobacco plants, whether infested withHelicoverpa armigeralarvae or not, significantly incited orientation behavior of parasitoid waspsMicroplitis mediator. CONCLUSION Wild typeN. tabacumplants have no DMNT metabolic pathway. DMNT could bede novobiosynthesized via co-expression ofGhCYP82Ls andGhTPS14. What is more, the parasitoid waspM. mediatorcould be recruited by DMNT-releasing transgenic tobaccos, especially byH. armigera-infested transgenic tobaccos, suggesting the potential roles of engineeredN. tabacumin regulating the behavioral preference ofM. mediator.

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