DROOPY LEAF1 controls leaf architecture by orchestrating early brassinosteroid signaling

文献类型： 外文期刊

第一作者： Zhao, Meicheng

作者： Zhao, Meicheng;Zhao, Meicheng;Tang, Sha;Zhang, Haoshan;He, Miaomiao;Liu, Jihong;Zhi, Hui;Sui, Yi;Liu, Xiaotong;Jia, Guanqing;Wu, Chuanyin;Diao, Xianmin;He, Miaomiao;Wang, Xingchun;Liu, Jihong;Zhao, Zhiying;Zhao, Baohua;Tang, Wenqiang;Liu, Xigang;Yan, Jijun;Zhang, Baocai;Zhou, Yihua;Chu, Jinfang;Li, Jiayang;Yan, Jijun;Zhang, Baocai;Zhou, Yihua;Chu, Jinfang;Li, Jiayang;Yan, Jijun;Zhang, Baocai;Zhou, Yihua;Chu, Jinfang;Li, Jiayang;Liu, Xigang

作者机构：

关键词： plant architecture; leaf droopiness; brassinosteroid signaling; negative feedback regulation

期刊名称：PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA （ 影响因子：11.205； 五年影响因子：12.291 ）

ISSN： 0027-8424

年卷期： 2020 年 117 卷 35 期

页码：

收录情况： SCI

摘要： Leaf architecture directly determines canopy structure, and thus, grain yield in crops. Leaf droopiness is an agronomic trait primarily affecting the cereal leaf architecture but the genetic basis and underlying molecular mechanism of this trait remain unclear. Here, we report that DROOPY LEAF1 (DPY1), an LRR receptor-like kinase, plays a crucial role in determining leaf droopiness by controlling the brassinosteroid (BR) signaling output in Setaria, an emerging model for Panicoideae grasses. Loss-of-function mutation in DPY1 led to malformation of vascular sclerenchyma and low lignin content in leaves, and thus, an extremely droopy leaf phenotype, consistent with its preferential expression in leaf vascular tissues. DPY1 interacts with and competes for SiBAK1 and as a result, causes a sequential reduction in SiBRI1-SiBAK1 interaction, SiBRI1 phosphorylation, and downstream BR signaling. Conversely, DPY1 accumulation and affinity of the DPY1-SiBAK1 interaction are enhanced under BR treatment, thus preventing SiBRI1 from overactivation. As such, those findings reveal a negative feedback mechanism that represses leaf droopiness by preventing an overresponse of early BR signaling to excess BRs. Notably, plants overexpressing DPY1 have more upright leaves, thicker stems, and bigger panicles, suggesting potential utilization for yield improvement. The maize ortholog of DPY1 rescues the droopy leaves in dpy1, suggesting its conserved function in Panicoideae. Together, our study provides insights into how BR signaling is scrutinized by DPY1 to ensure the upward leaf architecture.

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