Heat Shock Factor Is Involved in Regulating the Transcriptional Expression of Two Potential Hsps (AhHsp70andAhsHsp21) and Its Role in Heat Shock Response ofAgasicles hygrophila

文献类型： 外文期刊

第一作者： Jin, Jisu

作者： Jin, Jisu;Li, Youzhi;Wan, Fanghao;Jin, Jisu;Zhou, Zhongshi;Zhang, Hong;Guo, Jianying;Wan, Fanghao

作者机构：

关键词： Agasicles hygrophila; heat shock protein; heat shock factor; RNAi; thermotolerance

期刊名称：FRONTIERS IN PHYSIOLOGY （ 影响因子：4.566； 五年影响因子：4.804 ）

ISSN： 1664-042X

年卷期： 2020 年 11 卷

页码：

收录情况： SCI

摘要： Heat shock proteins are molecular chaperones that are involved in numerous normal cellular processes and stress responses, and heat shock factors are transcriptional activators of heat shock proteins. Heat shock factors and heat shock proteins are coordinated in various biological processes. The regulatory function of heat shock factors in the expression of genes encoding heat shock proteins (Hsps) has been documented in some model insects, however, the role of transcription factors in modulating Hsps in other insects is still limited. In this study, one heat shock factor gene (AhHsf) was isolated and its two potential target genes (AhHsp70andAhsHsp21) were confirmed fromAgasicles hygrophila.AhHsfsequence analysis indicated that it belongs to the Hsfs gene family. RT-qPCR showed that expression levels of heat shock factors and of two heat shock proteins significantly increased under heat stress. Injection with double-strandedHsfRNA in freshly emerged adult beetles significantly inhibited expression ofAhHsp70andAhsHsp21, shortened the adult survival, drastically reduced egg production, and ultimately led to a decrease in fecundity. RNA interference (RNAi)-mediated suppression ofAhHsp70orAhsHsp21expression also significantly affected expression ofAhHsf. Our findings revealed a potential transcriptional function of AhHsf to regulate expression ofAhHsp70andAhsHsp21, which may play a key role inA. hygrophilathermotolerance. Our results improve our understanding of the molecular mechanisms of the AhHsf - AhHsps signaling pathway inA. hygrophila.

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