Bioassay-guided isolation of two antifungal compounds from Magnolia officinalis, and the mechanism of action of honokiol

文献类型： 外文期刊

第一作者： Yan, Yin-Fang

作者： Yan, Yin-Fang;Yang, Cheng-Jie;Shang, Xiao-Fei;Zhao, Zhong-Min;Liu, Ying-Qian;Zhou, Rui;Liu, Hua;Wu, Tian-Lin;Zhao, Wen-Bin;Qin, Fang;He, Ying-Hui;Li, Hai-Xin;Du, Sha-Sha;Wang, Yu-Ling;Hu, Guan-Fang

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关键词： Magnolia officinalis; Honokiol; Antifungal activity; Rhizoctonia solani

期刊名称：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：3.963； 五年影响因子：4.454 ）

ISSN： 0048-3575

年卷期： 2020 年 170 卷

页码：

收录情况： SCI

摘要： Magnolia officinalis, as a well-known herb worldwide, has been widely used to treat multiple diseases for a long time. In this study, the petroleum ether extract from M. officinalis showed effective antifungal activity against seven plant pathogens (particularly against R. solani with an inhibition rate of 100.00% at 250 mu g/mL). Honokiol and magnolol, isolated by the bioassay-guided method, exhibited greater antifungal activity than tebuconazole (EC50 = 3.07 mu g/mL, p <= 0.001) against R. solani, which EC50 values were 2.18 mu g/mL and 3.48 mu g/mL, respectively. We used transcriptomics to explore the mechanism of action of honokiol against R. solani. Results indicated that honokiol may exert antifungal effects by blocking the oxidative phosphorylation metabolic pathway. Further studies indicated that honokiol induced ROS overproduction, disrupted the mitochondrial function, affected respiration, and blocked the TCA cycle, which eventually inhibited ATP production. Besides, honokiol also damaged cell membranes and caused morphological changes. This study demonstrated that the lignans isolated from M. officinalis possess the potential to be developed as botanical fungicides.

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