Loci and candidate gene identification for soybean resistance to Phytophthora root rot race 1 in combination with association and linkage mapping

文献类型： 外文期刊

第一作者： Zhao, Xue

作者： Zhao, Xue;Bao, Dongfang;Wang, Wei;Zhang, Chanjuan;Jing, Yan;Jiang, Haipeng;Li, Wenbin;Han, Yingpeng;Qiu, Lijuan

作者机构：

关键词： Association analysis; Candidate genes; Linkage analysis; Phytophthora sojaeresistance; Soybean

期刊名称：MOLECULAR BREEDING （ 影响因子：2.589； 五年影响因子：2.75 ）

ISSN： 1380-3743

年卷期： 2020 年 40 卷 10 期

页码：

收录情况： SCI

摘要： As the second most destructive disease in soybean (Glycine max(L.) Merr), Phytophthora root or stem-rot (PRR) of soybean, which was caused byPhytophthora sojae(P.sojae), could lead to a 10-40% or complete yield loss annually. Researches were needed to perform to identify theP.sojae-resistant germplasm and to better understand the genetic basis ofP. sojaeresistance in soybean. A total of 225 diverse soybean cultivars and 109 recombinant inbred lines (RILs) derived from crossing 'DongnongL-28' (resistant toP.sojaerace 1) and 'Hefeng 25' (susceptible toP.sojaerace 1) were used to evaluateP.sojaerace 1 resistance. These 225 soybean cultivars were sequenced using the Specific Locus Amplified Fragment Sequencing (SLAF-seq) approach, and 28,722 single-nucleotide polymorphisms (SNPs) were obtained from the mapping of resistant loci through genome-wide association. Eight quantitative trait nucleotides (QTNs) were associated with resistance toP.sojaerace 1. The QTN on Chr.03 was colocalised according to linkage mapping of the RILs. A total of 18 candidate genes were predicted in the flanking region of the colocalised locus on Chr.03. There were stress response-related motifs, such as cis-acting regulatory elements involved in salicylic acid or MeJA responsiveness, in the 1-kb upstream region of sixteen genes. Quantitative RT-PCR showed that theGlyma.03G033700was induced byP.sojaerace 1. Association analysis for Rps loci showed thatGlyma.03G033700andGlyma.03G033800were the candidates near peak SNP. The identified loci along with the candidate genes could be valuable for studying the molecular mechanisms underlying soybean resistance toP.sojaerace 1 and breeding resistant varieties.

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