Construction of the recombinant duck enteritis virus delivering capsid protein VPO of the duck hepatitis A virus
文献类型： 外文期刊
第一作者： Niu, Yinjie
作者： Niu, Yinjie;Liu, Baihan;Sun, Chang;Zhao, Lili;Chen, Hongyan
作者机构：
关键词： Duck hepatitis A virus; Duck enteritis virus; VPO; Protection; ul41
期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：3.293； 五年影响因子：3.599 ）
ISSN： 0378-1135
年卷期： 2020 年 249 卷
页码：
收录情况： SCI
摘要： Duck hepatitis A virus type 1 (DHAV-1) disease causes significant economic losses to the duck industry. Duck enteritis virus (DEV) is frequently used as a viral vector for aquatic poultry vaccination, but no recombinan DEV expressing DHAV-1 VPO has been developed. In this study, we established a system for rescuing the DEV C-KCE vaccine strain by transfecting cells with six fosmid DNAs. We generated a recombinant virus (rDEV-ul41VPO) by inserting the VPO gene of DHAV-1 into the ul41 region in the DEV C-KCE genome. DHAV-1 VPO was stably expressed in the rDEV-ul41VPO infected cells, but did not affect the replication properties of DEV C-KCE in cells. Duck experiments showed that rDEV-ul41VPO could provided full protection against the lethal DEV Chinese standard challenge (DEV CSC) and conferred 70% protection against DHAV-1 161/79 at 3 days postvaccination. These results indicate that rDEV-ul41VPO rapidly induces protection against DEV CSC and DHAV-1 in ducks, and can be served as a bivalent vaccine against DEV and DHAV-1.
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