Exocyst subunitBcSec3regulates growth, development and pathogenicity inBotrytis cinerea

文献类型： 外文期刊

第一作者： Ma, Zhiwei

作者： Ma, Zhiwei;Chen, Zhixiong;Wang, Kun;Zhu, Tingheng;Wang, Weixia

作者机构：

关键词： Botrytis cinerea; BcSec3; growth; pathogenicity

期刊名称：JOURNAL OF BIOSCIENCES （ 影响因子：1.826； 五年影响因子：2.107 ）

ISSN： 0250-5991

年卷期： 2020 年 45 卷 1 期

页码：

收录情况： SCI

摘要： Botrytis cinereais a saprophytic plant pathogenic fungus that can infect a variety of crops and cause gray mold, which leads to huge losses worldwide. The role of exocyst in fungal pathogenicity is being revealed. In this study, homologous recombination technology was used to knock out the exocyst subunitBcSec3ofB. cinerea, and it was found that theBcSec3subunit plays a crucial role in the growth and pathogenicity ofB. cinerea. Compared with the wild-type strain B05.10, the mycelial growth ability of theBcSec3deletion strain was reduced by up to 49.8%, the conidia production capacity of the deletion strain was severely lost, and no sclerotia was formed. The polygalacturonase, is one of plant cell wall hydrolases, whose activity inBcSec3deletion strain was significantly reduced. In the tomato leaves infection assayin vitro, the lesion area caused by theBcSec3deletion strain was only 20% of the wild type after 5 days of infection. Observation by light microscope showed that the morphology ofBcSec3deletion strain mycelium was significantly changed, the mycelium became thinner and deformed, and the polarity growth was not obvious. Further observation with laser confocal microscopy and transmission electron microscopy was conducted. It was found that compared with the wild type, the number of vesicles inBcSec3deleted cells reduced and localization and distribution of vesicles changed. In mutant cell, vesicles relatively concentrated in the cytoplasm, while in wild-type cell mainly concentrated inside the cell membrane. These evidences indicate that the exocyst subunitBcSec3plays an important role in the growth, development and pathogenicity ofB. cinerea.

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