Two-step enzymatic synthesis of alpha-linolenic acid-enriched diacylglycerols with high purities from silkworm pupae oil

文献类型： 外文期刊

第一作者： Liu, Xuan

作者： Liu, Xuan;Xu, Long;Lan, Dongming;Wang, Yonghua;Shi, Wei;Liao, Sentai;Wang, Weifei;Yang, Bo

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关键词： Silkworm pupae oil; Diacylglycerol; α -Linolenic acid; Biocatalysis; Esterification

期刊名称：BIOPROCESS AND BIOSYSTEMS ENGINEERING （ 影响因子：3.21； 五年影响因子：2.97 ）

ISSN： 1615-7591

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收录情况： SCI

摘要： In this study, alpha-linolenic acid-enriched diacylglycerols (ALA-DAGs) were prepared via a two-step enzymatic way by combi-lipase using silkworm pupae oils as substrates. Firstly, several factors including temperature, mass ratio of water to oil, pH and enzyme loading were optimized for the hydrolysis of silkworm pupae oil. The maximum fatty acid content (96.51%) was obtained under the conditions: temperature 40 degrees C, water/oil 3:2 (w/w), pH 7, lipase TL100L loading 400 U/g, lipase PCL loading 30 U/g. Then, ALA was enriched by urea inclusion, with an increased ALA content of 82.50% being obtained. Secondly, the ALA-enriched silkworm pupae DAG oil (SPDO) was prepared by lipase PCL-catalyzed esterification reaction. After molecular distillation, the final SPDO product contained contents of DAGs (97.01%) and ALA (82.50%). This two-step enzymatic way for production of ALA-DAGs was successfully applied in a 100-fold scale-up reaction. Overall, our study provides a promising way for the preparation of ALA-DAGs.

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