Dynamic Covalent Hydrogel as a Single-Dose Vaccine Adjuvant for Sustained Antigen Release and Significantly Elevated Humoral Immunity

文献类型： 外文期刊

第一作者： Shi, Zhiyuan

作者： Shi, Zhiyuan;Huang, Zichao;Song, Wantong;Chen, Xuesi;Shi, Zhiyuan;Si, Xinghui;Huang, Zichao;Ma, Sheng;Guo, Zhaopei;Song, Wantong;Chen, Xuesi;Gao, Zihan;Zhuang, Xinyu;Di, Yaxin;Li, Chang;Jin, Ningyi;Tian, Mingyao;Si, Xinghui;Ma, Sheng;Guo, Zhaopei;Song, Wantong;Chen, Xuesi;Huang, Leaf

作者机构：

关键词： hydrogel; nipah; SARS-CoV-2; vaccine

期刊名称：ADVANCED HEALTHCARE MATERIALS （ 影响因子：10.0； 五年影响因子：11.3 ）

ISSN： 2192-2640

年卷期： 2024 年

页码：

收录情况： SCI

摘要： Vaccine is the most important way for fighting against infection diseases. However, multiple injections and unsatisfied immune responses are the main obstacles for current vaccine application. Herein, a dynamic covalent hydrogel (DCH) is used as a single-dose vaccine adjuvant for eliciting robust and sustained humoral immunity. By adjusting the mass ratio of the DCH gel, 10-30 d constant release of the loaded recombinant protein antigens is successfully realized, and it is proved that sustained release of antigens can significantly improve the vaccine efficacy. When loading SARS-CoV-2 RBD (Wuhan and Omicron BA.1 strains) antigens into this DCH gel, an over 32 000 times and 8000 times improvement is observed in antigen-specific antibody titers compared to conventional Aluminum adjuvanted vaccines. The universality of this DCH gel adjuvant is confirmed in a Nipah G antigen test as well as a H1N1 influenza virus antigen test, with much improved protection of C57BL/6 mice against H1N1 virus infection than conventional Aluminum adjuvanted vaccines. This sustainably released, single-dose DCH gel adjuvant provides a new promising option for designing next-generation infection vaccines. Using a dynamic covalent hydrogel (DCH) technology, a sustained antigen release from 10 to 30 d after subcutaneous injection can be realized, and this kind of sustained release is able to significantly elevate the antibody titer levels with OVA (37 640x), Wuhan-RBD (32 000x), Omicron-RBD (8200x), and Nipah-G (1300x) antigens than the conventional Aluminum adjuvant, as well as an excellent protection effect for H1N1 viral infection. In addition, the DCH gel-based vaccine is carried out only once, which can simplify the vaccination process. image

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