Magnetic Relaxation Switching Immunoassay Based on Hydrogen Peroxide-Mediated Assembly of Ag@Au-Fe3O4 Nanoprobe for Detection of Aflatoxin B1

文献类型： 外文期刊

第一作者： Dong, Yongzhen

作者： Dong, Yongzhen;Chen, Yiping;Wen, Cong-ying;Zhang, Yu;Zeng, Jingbin;She, Yongxin

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关键词： aflatoxin B1; Ag@Au-Fe; O-3; (4)-MRS immunosensor; dumbbell-like Au-Fe; O-3; (4) nanoparticles; hydrogen peroxide; magnetic relaxation switching

期刊名称：SMALL （ 影响因子：13.281； 五年影响因子：12.463 ）

ISSN： 1613-6810

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收录情况： SCI

摘要： Magnetic relaxation switching (MRS) sensors have shown great potential in food safety monitoring due to their high signal-to-noise ratio and simplicity, but they often suffer from insufficient sensitivity and stability due to the lack of excellent magnetic nanoprobes. Herein, dumbbell-like Au-Fe3O4 nanoparticles are designed as magnetic nanoprobes for developing an aflatoxin B1-MRS immunosensor. The Fe3O4 portion in the Au-Fe3O4 nanoparticles functions as the magnetic probe to provide transverse relaxation signals, while the Au segments serve as a bridge to grow Ag shell and assemble the Au-Fe3O4 nanoparticles, thus modulating transverse relaxation time of surrounding water molecular. The formation of Ag@Au-Fe3O4 is triggered by hydrogen peroxide. After degraded by horseradish peroxidase, hydrogen peroxide reduces Ag+ to Ag nanoparticles which assemble dispersed Au-Fe3O4 to aggregated Ag@Au-Fe3O4, thus dramatically improving the sensitivity of traditional MRS sensor. Combined with competitive immunoreaction, this Ag@Au-Fe3O4-MRS immunosensor can detect aflatoxin B1 with a high sensitivity (3.81 pg mL(-1)), which improved about 21 folds and 9 folds than those of enzyme-linked immunosorbent assay and high-performance liquid chromatography (HPLC), respectively. The good consistency with HPLC in real samples detection indicates the good accuracy of this immunosensor. This Ag@Au-Fe3O4-MRS immunosensor offers an attractive tool for detection of harmful substances.

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